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Incorporation of oxygen into the succinate co-product of iron(II) and 2-oxoglutarate dependent oxygenases from bacteria, plants and humans.

Welford, Richard W.D., Kirkpatrick, Joanna M., McNeill Luke A., Puri, Munish, Oldham, Neil J. and Schofield, Christopher J. 2005, Incorporation of oxygen into the succinate co-product of iron(II) and 2-oxoglutarate dependent oxygenases from bacteria, plants and humans., FEBS Letters, vol. 579, no. 23, pp. 5170-5174.

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Title Incorporation of oxygen into the succinate co-product of iron(II) and 2-oxoglutarate dependent oxygenases from bacteria, plants and humans.
Author(s) Welford, Richard W.D.
Kirkpatrick, Joanna M.
McNeill Luke A.
Puri, Munish
Oldham, Neil J.
Schofield, Christopher J.
Journal name FEBS Letters
Volume number 579
Issue number 23
Start page 5170
End page 5174
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2005-09-26
ISSN 0014-5793
1873-3468
Keyword(s) hypoxia-inducible factor
hydroxylase
2-Oxoglutarate
prolyl hydroxylase
succinate
Summary The ferrous iron and 2-oxoglutarate (2OG) dependent oxygenases catalyse two electron oxidation reactions by coupling the oxidation of substrate to the oxidative decarboxylation of 2OG, giving succinate and carbon dioxide coproducts. The evidence available on the level of incorporation of one atom from dioxygen into succinate is inconclusive. Here, we demonstrate that five members of the 2OG oxygenase family, AlkB from Escherichia coli, anthocyanidin synthase and flavonol synthase from Arabidopsis thaliana, and prolyl hydroxylase domain enzyme 2 and factor inhibiting hypoxia-inducible factor-1 from Homo sapiens all incorporate a single oxygen atom, almost exclusively derived from dioxygen, into the succinate co-product.
Language eng
Field of Research 060112 Structural Biology (incl Macromolecular Modelling)
100301 Biocatalysis and Enzyme Technology
030406 Proteins and Peptides
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2005, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019701

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Created: Fri, 18 Sep 2009, 16:02:46 EST by Munish Puri