Production of high fructose syrup from asparagus inulin using immobilized exoinulinase from Kluyveromyces marxianus YS-1

Singh, Ram Sarup, Dhaliwal, Rajesh and Puri, Munish 2007, Production of high fructose syrup from asparagus inulin using immobilized exoinulinase from Kluyveromyces marxianus YS-1, Journal of industrial microbiology and biotechnology, vol. 34, no. 10, pp. 649-655.

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Title Production of high fructose syrup from asparagus inulin using immobilized exoinulinase from Kluyveromyces marxianus YS-1
Formatted title Production of high fructose syrup from Asparagus inulin using immobilized exoinulinase from Kluyveromyces marxianus YS-1
Author(s) Singh, Ram Sarup
Dhaliwal, Rajesh
Puri, Munish
Journal name Journal of industrial microbiology and biotechnology
Volume number 34
Issue number 10
Start page 649
End page 655
Publisher Springer
Place of publication The Netherlands
Publication date 2007-07-31
ISSN 1367-5435
Keyword(s) Kluyveromyces marxianus
inulinase
immobilization
high fructose syrup
Summary Extracellular exoinulinase from Kluyveromyces marxianus YS-1, which hydrolyzes inulin into fructose, was immobilized on Duolite A568 after partial puriWcation by ethanol precipitation and gel exclusion chromatography on Sephadex G-100. Optimum temperature of immobilized enzyme was 55 °C, which was 5 °C higher than the free enzyme and optimal pH was 5.5. Immobilized biocatalyst retained more than 90% of its original activity after incubation at 60 °C for 3 h, whereas in free form its activity was reduced to 10% under same conditions, showing a signiWcant improvement in the thermal stability of the biocatalyst after immobilization. Apparent Km values for inulin, raYnose and sucrose were found to be 3.75, 28.5 and 30.7 mM, respectively. Activation energy (Ea) of the immobilized biocatalyst was found to be 46.8 kJ/mol. Metal ions like Co2+ and Mn2+ enhanced the activity, whereas Hg2+ and Ag2+ were found to be potent inhibitors even at lower concentrations of 1 mM. Immobilized biocatalyst was eVectively used in batch preparation of high fructose syrup from Asparagus racemosus raw inulin and pure inulin, which
yielded 39.2 and 40.2 g/L of fructose in 4 h; it was 85.5 and 92.6% of total reducing sugars produced, respectively.
Language eng
Field of Research 060107 Enzymes
100302 Bioprocessing, Bioproduction and Bioproducts
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2007, Society for Industrial Microbiology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019709

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Created: Fri, 18 Sep 2009, 17:11:38 EST by Munish Puri

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