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Partial purification and characterization of limonoate dehydrogenase from Rhodococcus fascians for the degradation of limonin

Puri, Munish, Kaur, Lakhwinder and Marwaha, Satwinder Singh 2002, Partial purification and characterization of limonoate dehydrogenase from Rhodococcus fascians for the degradation of limonin, Journal of microbiology and biotechnology, vol. 12, no. 4, pp. 669-673.

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Title Partial purification and characterization of limonoate dehydrogenase from Rhodococcus fascians for the degradation of limonin
Formatted title Partial purification and characterization of limonoate dehydrogenase from Rhodococcus fascians for the degradation of limonin
Author(s) Puri, Munish
Kaur, Lakhwinder
Marwaha, Satwinder Singh
Journal name Journal of microbiology and biotechnology
Volume number 12
Issue number 4
Start page 669
End page 673
Publisher Korean Society for Microbiology and Biotechnology
Place of publication Korea
Publication date 2002-08
ISSN 1017-7825
1598-642X
1738-8872
Keyword(s) purification
limonoate dehydrogenase
limonin
debittering
R. fascians
Kinnow mandarin juice
Summary An extracellular limonoate dehydrogenase was purified 10-fold from a cell-free extract of Rhodococcus fascians by ammonium sulfate precipitation, dialysis, and ultrafiltration. This purified dehydrogenase catalyzed the
conversion of limonoate to 17-dehydrolimonoate. The enzyme showed optimum activity at pH 8.0 and 40oC, with Km value of 0.9 µM, and requires Zn ions and sulfhydryl groups for catalytic action. The enzyme activity was inhibited by Hg2+ and NaN3 ions. The degradation of limonin (66%) in Kinnow mandarin juice was successfully demonstrated with partially
purified limonoate dehydrogenase. With scale-up preparation of limonoate dehydrogenase, a successful debittering operation of fruit juices appears feasible.
Notes
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Language eng
Field of Research 100399 Industrial Biotechnology not elsewhere classified
100301 Biocatalysis and Enzyme Technology
100302 Bioprocessing, Bioproduction and Bioproducts
Socio Economic Objective 970110 Expanding Knowledge in Technology
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2002, The Korean Society of Microbiology and Biotechnology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019711

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Created: Fri, 18 Sep 2009, 17:31:14 EST by Munish Puri