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Response surface optimisation of medium components for naringinase production from staphylococcus xylosus MAK 2

Puri, Munish, Kaur, Aneet, Singh, Ram Sarup and Singh, Anubhav 2010, Response surface optimisation of medium components for naringinase production from staphylococcus xylosus MAK 2, Applied biochemistry and biotechnology, vol. 162, no. 1, pp. 181-191.

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Title Response surface optimisation of medium components for naringinase production from staphylococcus xylosus MAK 2
Formatted title Response surface optimisation of medium components for naringinase production from Staphylococcus xylosus MAK 2
Author(s) Puri, Munish
Kaur, Aneet
Singh, Ram Sarup
Singh, Anubhav
Journal name Applied biochemistry and biotechnology
Volume number 162
Issue number 1
Start page 181
End page 191
Total pages 11
Publisher Humana Press
Place of publication Totowa, United States
Publication date 2010-09
ISSN 0273-2289
1073-6085
1559-0291
Keyword(s) response surface methodology
central composite rotatable design
naringin
Staphylococcus xylosus
naringinase
Summary Response surface methodology was used to optimize the fermentation medium for enhancing naringinase production by Staphylococcus xylosus. The first step of this process involved the individual adjustment and optimization of various medium components at shake flask level. Sources of carbon (sucrose) and nitrogen (sodium nitrate), as well as an inducer (naringin) and pH levels were all found to be the important factors significantly affecting naringinase production. In the second step, a 22 full factorial central composite design was applied to determine the optimal levels of each of the significant variables. A second-order polynomial was derived by multiple regression analysis on the experimental data. Using this methodology, the optimum values for the critical components were obtained as follows: sucrose, 10.0%; sodium nitrate, 10.0%; pH 5.6; biomass concentration, 1.58%; and naringin, 0.50% (w/v), respectively. Under optimal conditions, the experimental naringinase production was 8.45 U/mL. The determination coefficients (R 2) were 0.9908 and 0.9950 for naringinase activity and biomass production, respectively, indicating an adequate degree of reliability in the model.
Notes Published online: 8 September 2009
Language eng
Field of Research 060107 Enzymes
100302 Bioprocessing, Bioproduction and Bioproducts
Socio Economic Objective 860699 Industrial Chemicals and Related Products not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
HERDC collection year 2010
Copyright notice ©2009, Humana Press
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019714

Document type: Journal Article
Collection: Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin)
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Created: Fri, 18 Sep 2009, 18:15:02 EST by Munish Puri