Development of a stable continuous flow immobilized enzyme reactor for the hydrolysis of inulin

Puri, Munish, Singh, Ram Sarup and Dhaliwal, Rajesh 2008, Development of a stable continuous flow immobilized enzyme reactor for the hydrolysis of inulin, Journal of industrial microbiology & biotechnology, vol. 35, no. 7, pp. 777-782.

Attached Files
Name Description MIMEType Size Downloads

Title Development of a stable continuous flow immobilized enzyme reactor for the hydrolysis of inulin
Author(s) Puri, Munish
Singh, Ram Sarup
Dhaliwal, Rajesh
Journal name Journal of industrial microbiology & biotechnology
Volume number 35
Issue number 7
Start page 777
End page 782
Total pages 6
Publisher Springer
Place of publication Berlin, Germany
Publication date 2008-07
ISSN 1367-5435
1476-5535
Keyword(s) kluyveromyces marxianus
exoinulinase
immobilized enzyme reactor
inulin hydrolysis
Summary A 23.5-fold purified exoinulinase with a specific activity of 413 IU/mg and covalently immobilized on Duolite A568 has been used for the development of a continuous flow immobilized enzyme reactor for the hydrolysis of inulin. In a packed bed reactor containing 72 IU of exoinulinase from Kluyveromyces marxianus YS-1, inulin solution (5%, pH 5.5) with a flow rate of 4 mL/h was completely hydrolyzed at 55 °C. The reactor was run continuously for 75 days and its experimental half-life was 72 days under the optimized operational conditions. The volumetric productivity and fructose yield of the reactor were 44.5 g reducing sugars/L/h and 53.3 g/L, respectively. The hydrolyzed product was a mixture of fructose (95.8%) and glucose (4.2%) having an average fructose/glucose ratio of 24. An attempt has also been made to substitute pure inulin with raw Asparagus racemosus inulin to determine the operational stability of the developed reactor. The system remained operational only for 11 days, where 85.9% hydrolysis of raw inulin was achieved.
Notes Published online: 4 April 2008
Language eng
Field of Research 109999 Technology not elsewhere classified
Socio Economic Objective 970110 Expanding Knowledge in Technology
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2008, Society for Industrial Microbiology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019728

Document type: Journal Article
Collection: Institute of Biotechnology
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 11 times in TR Web of Science
Scopus Citation Count Cited 12 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 407 Abstract Views, 0 File Downloads  -  Detailed Statistics
Created: Mon, 21 Sep 2009, 10:36:01 EST by Liz Hau

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.