Overview of the pipeline for structural and functional characterization of macrophage proteins at the University of Queensland.

Meng, Weining, Forwood, Jade K., Guncar, Gregor, Robin, Gautier, Cowieson, Nathan P., Listwan, Pawel, Mouradov, Dmitri, King, Gordon, Ross, Ian L., Robinson, Jodie, Puri, Munish, Hill, Justine M., Kellie, Stuart, Huber, Thomas, Hume, David A., Martin, Jennifer L. and Kobe, Bostjan 2008, Overview of the pipeline for structural and functional characterization of macrophage proteins at the University of Queensland., in Structural proteomics : high-throughput methods, Humana Press, Clifton, N.J., pp.577-587.

Attached Files
Name Description MIMEType Size Downloads

Title Overview of the pipeline for structural and functional characterization of macrophage proteins at the University of Queensland.
Author(s) Meng, Weining
Forwood, Jade K.
Guncar, Gregor
Robin, Gautier
Cowieson, Nathan P.
Listwan, Pawel
Mouradov, Dmitri
King, Gordon
Ross, Ian L.
Robinson, Jodie
Puri, Munish
Hill, Justine M.
Kellie, Stuart
Huber, Thomas
Hume, David A.
Martin, Jennifer L.
Kobe, Bostjan
Title of book Structural proteomics : high-throughput methods
Editor(s) Hobe, Bostjan
Guss, Mitchell
Huber, Thomas
Publication date 2008
Series Methods in molecular biology ; 426
Chapter number 38
Total chapters 39
Start page 577
End page 587
Total pages 11
Publisher Humana Press
Place of Publication Clifton, N.J.
Summary This chapter describes the methodology adopted in a project aimed at structural and functional characterization of proteins that potentially play an important role in mammalian macrophages. The methodology that underpins this project is applicable to both small research groups and larger structural genomics consortia. Gene products with putative roles in macrophage function are identified using gene expression information obtained via DNA microarray technology. Specific targets for structural and functional characterization are then selected based on a set of criteria aimed at maximizing insight into function. The target proteins are cloned using a modification of Gateway® cloning technology, expressed with hexa-histidine tags in E. coli, and purified to homogeneity using a combination of affinity and size exclusion chromatography. Purified proteins are finally subjected to crystallization trials and/or NMR-based screening to identify candidates for structure determination. Where crystallography and NMR approaches are unsuccessful, chemical cross-linking is employed to obtain structural information. This resulting structural information is used to guide cell biology experiments to further investigate the cellular and molecular function of the targets in macrophage biology. Jointly, the data sheds light on the molecular and cellular functions of macrophage proteins.
ISBN 9781588298096
ISSN 1064-3745
Language eng
Field of Research 109999 Technology not elsewhere classified
Socio Economic Objective 970110 Expanding Knowledge in Technology
HERDC Research category B1.1 Book chapter
ERA Research output type B Book chapter
HERDC collection year 2008
Copyright notice ©2008, Humana Press
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019734

Document type: Book Chapter
Collection: Institute of Biotechnology
Connect to link resolver
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Version Filter Type
Citation counts: Scopus Citation Count Cited 2 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 407 Abstract Views, 1 File Downloads  -  Detailed Statistics
Created: Mon, 21 Sep 2009, 11:34:02 EST by Liz Hau

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.