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Partial purification and characterization of exoinulinase from kluyveromyces marxianus YS-1 for preparation of high-fructose syrup

Singh, Ram Sarup, Dhaliwal, Rajesh and Puri, Munish 2007, Partial purification and characterization of exoinulinase from kluyveromyces marxianus YS-1 for preparation of high-fructose syrup, Journal of microbiology and biotechnology, vol. 17, no. 5, pp. 733-738.

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Title Partial purification and characterization of exoinulinase from kluyveromyces marxianus YS-1 for preparation of high-fructose syrup
Author(s) Singh, Ram Sarup
Dhaliwal, Rajesh
Puri, Munish
Journal name Journal of microbiology and biotechnology
Volume number 17
Issue number 5
Start page 733
End page 738
Publisher Korean Society for Microbiology Biotechnology
Place of publication Seoul, Korea
Publication date 2007-05
ISSN 1017-7825
1598-642X
1738-8872
Keyword(s) Kluyveromyces marxianus
Asparagus racemosus
exoinulinase
partial purification
high-fructose syrup
Summary An extracellular exoinulinase( 2, 1- ß- D fructan fructanohydrolase, EC 3.2.1.7), which catalyzes the hydrolysis of inulin into fructose and glucose, was purified 23.5-fold by ethanol precipitation, followed by Sephadex G-100 gel permeation from a cell-free extract of Kluyveromyces marxianus YS-1. The partially purified enzyme exhibited considerable activity between pH 5 to 6, with an optimum pH of 5.5, while it remained stable(100%) for 3 h at the optimum temperature of 50º c. Mn2+ and Ca2+ produced a 2A-fold and 1.2-fold enhancement in enzyme activity, whereas Hg2+ and Ag2+  completely inhibited the inulinase. A preparation of the partially purified enzyme effectively hydrolyzed inulin, sucrose, and raffinose, yet no activity was found with starch, lactose, and maltose. The enzyme preparation was then successfully used to hydrolyze pure inulin and raw inulin from Asparagus racemosus for the preparation of a high-fructose syrup. In a batch system, the exoinulinase hydrolyzed 84.8% of the pure inulin and 86.7% of the raw Asparagus racemosus inulin, where fructose represented 43.6mg/ml and 41.3mg/ml, respectively.
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Language eng
Field of Research 109999 Technology not elsewhere classified
Socio Economic Objective 970110 Expanding Knowledge in Technology
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2007, Korean Society for Microbiology Biotechnology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019736

Document type: Journal Article
Collections: Institute of Biotechnology
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