Optimization of medium and process parameters for the production of inulinase from a newly isolated Kluyveromyces marxianus YS-1

Sooch, Balwinder Singh, Singh, R. S. and Puri, Munish 2007, Optimization of medium and process parameters for the production of inulinase from a newly isolated Kluyveromyces marxianus YS-1, Bioresource technology, vol. 98, no. 13, pp. 2518-2525.

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Title Optimization of medium and process parameters for the production of inulinase from a newly isolated Kluyveromyces marxianus YS-1
Formatted title Optimization of medium and process parameters for the production of inulinase from a newly isolated Kluyveromyces marxianus YS-1
Author(s) Sooch, Balwinder Singh
Singh, R. S.
Puri, Munish
Journal name Bioresource technology
Volume number 98
Issue number 13
Start page 2518
End page 2525
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2007-09
ISSN 0960-8524
1873-2976
Keyword(s) inulin
inulinase
kluyveromyces marxianus
medium optimization
Summary A newly isolated strain of Kluyveromyces marxianus YS-1 was used for the production of extra cellular inulinase in a medium containing inulin, meat extract, CaCl2 and sodium dodecyl sulphate (SDS). Fermentation medium pH 6.5, cultivation temperature 30 °C and 5% (v/v) inoculum of 12 h-old culture were optimal for enzyme production (30.8 IU/ml) with a fermentation time of 72 h at shake flask level. Raw inulin (2%, w/v) extracted from dahlia tubers by processing at 15 kg/cm2 for 10 min was optimum for bioreactor studies. Maximum enzyme production (55.4 IU/ml) was obtained at an agitation rate of 200 rpm and aeration of 0.75 vvm in a stirred tank reactor with a fermentation time of 60 h.
Language eng
Field of Research 109999 Technology not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2006, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019739

Document type: Journal Article
Collection: Institute of Biotechnology
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