Focusing in on structural genomics : the University of Queensland structural biology pipeline

Puri, Munish, Robin, Gautier, Cowieson, Nathan, Forwood, Jade K., Listwan, Pawel, Hua, Shu-Hong, Guncar, Gregor, Huber, Thomas, Kellie, Stuart, Hume, David A., Kobe, Bostjan and Martin, Jennifer L. 2006, Focusing in on structural genomics : the University of Queensland structural biology pipeline, Biomolecular engineering, vol. 23, no. 6, pp. 281-289, doi: 10.1016/j.bioeng.2006.09.002.

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Title Focusing in on structural genomics : the University of Queensland structural biology pipeline
Author(s) Puri, MunishORCID iD for Puri, Munish
Robin, Gautier
Cowieson, Nathan
Forwood, Jade K.
Listwan, Pawel
Hua, Shu-Hong
Guncar, Gregor
Huber, Thomas
Kellie, Stuart
Hume, David A.
Kobe, Bostjan
Martin, Jennifer L.
Journal name Biomolecular engineering
Volume number 23
Issue number 6
Start page 281
End page 289
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2006-12
ISSN 1389-0344
Keyword(s) high throughput crystallography
protein expression
macrophage proteins
structural genomics
Summary The flood of new genomic sequence information together with technological innovations in protein structure determination have led to worldwide structural genomics (SG) initiatives. The goals of SG initiatives are to accelerate the process of protein structure determination, to fill in protein fold space and to provide information about the function of uncharacterized proteins. In the long-term, these outcomes are likely to impact on medical biotechnology and drug discovery, leading to a better understanding of disease as well as the development of new therapeutics. Here we describe the high throughput pipeline established at the University of Queensland in Australia. In this focused pipeline, the targets for structure determination are proteins that are expressed in mouse macrophage cells and that are inferred to have a role in innate immunity. The aim is to characterize the molecular structure and the biochemical and cellular function of these targets by using a parallel processing pipeline. The pipeline is designed to work with tens to hundreds of target gene products and comprises target selection, cloning, expression, purification, crystallization and structure determination. The structures from this pipeline will provide insights into the function of previously uncharacterized macrophage proteins and could lead to the validation of new drug targets for chronic obstructive pulmonary disease and arthritis.
Language eng
DOI 10.1016/j.bioeng.2006.09.002
Field of Research 109999 Technology not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2006 Elsevier B.V.
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