Production of inulinase from Kluyveromyces marxianus YS-1 using root extract of Asparagus racemosus

Singh, R. S. and Puri, Munish 2006, Production of inulinase from Kluyveromyces marxianus YS-1 using root extract of Asparagus racemosus, Process biochemistry, vol. 41, no. 7, pp. 1703-1707.


Title Production of inulinase from Kluyveromyces marxianus YS-1 using root extract of Asparagus racemosus
Formatted title Production of inulinase from Kluyveromyces marxianus YS-1 using root extract of Asparagus racemosus
Author(s) Singh, R. S.
Puri, Munish
Journal name Process biochemistry
Volume number 41
Issue number 7
Start page 1703
End page 1707
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2006-07
ISSN 1359-5113
1873-3298
Keyword(s) Kluyveromyces marxianus YS-1
Asparagus racemosus
root extract
inulinase
production
optimization
Summary Roots of Asparagus racemosus were used as a source of inulin for the production of inulinase from Kluyveromyces marxianus YS-1. Root extract prepared at 10 kg/cm2 for 10 min showed the maximum production of inulinase. Various parameters of fermentation have been examined in order to improve overall enzyme yield. Inulinase yield of 40.5 IU/mL in an optimized medium containing inulin (3.5%), beef extract (0.5%), MnSO4 (0.5 mM), CoCl2 (0.05 mM), SDS (0.4 mM) and pH 6.5 at 30 °C under agitation (150 rpm) for 60 h has been obtained at shake flask level. After optimization the enzyme production has been increased 6.1 times at flask level. In a bioreactor (working volume 1 L) fermentation medium containing 4% inulin has shown maximum inulinase production (47.3 IU/mL) under optimized parameters of 200 rpm, 0.75 vvm aeration at 30 °C after 60 h.
Language eng
Field of Research 109999 Technology not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2006, Elsevier Ltd
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019741

Document type: Journal Article
Collection: Institute of Biotechnology
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 25 times in TR Web of Science
Scopus Citation Count Cited 32 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 404 Abstract Views  -  Detailed Statistics
Created: Mon, 21 Sep 2009, 15:07:41 EST by Liz Hau

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.