Identification of secreted proteins associated with obesity and type 2 diabetes in Psammomys obesus

Bolton, K, Segal, D, McMillan, J, Sanigorski, A, Collier, G and Walder, K 2009, Identification of secreted proteins associated with obesity and type 2 diabetes in Psammomys obesus, International journal of obesity, vol. 33, pp. 1-13.

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Title Identification of secreted proteins associated with obesity and type 2 diabetes in Psammomys obesus
Author(s) Bolton, K
Segal, D
McMillan, J
Sanigorski, A
Collier, G
Walder, K
Journal name International journal of obesity
Volume number 33
Start page 1
End page 13
Publisher Nature Publishing Group
Place of publication Hampshire, England
Publication date 2009-07-28
ISSN 0307-0565
1476-5497
Keyword(s) signal sequence trap
secreted proteins
psammomys obesus
periostin
type 2 diabetes
diabetes
obesity
Summary Objective: Skeletal muscle produces a variety of secreted proteins that have important roles in intercellular communication and affects processes such as glucose homoeostasis. The objective of this study was to develop a novel Signal Sequence Trap (SST) in conjunction with cDNA microarray technology to identify proteins secreted from skeletal muscle of Psammomys obesus that were associated with obesity and type 2 diabetes (T2D).

Design: Secreted proteins that were differentially expressed between lean, normal glucose tolerant (NGT), overweight and impaired glucose tolerant (IGT) and obese, T2D P. obesus were isolated using SST in conjunction with cDNA microarray technology. Subsequent gene expression was measured in tissues from P. obesus by real-time PCR (RT-PCR).

Results: The SST yielded 1600 positive clones, which were screened for differential expression. A total of 91 (B6%) clones were identified by microarray to be differentially expressed between NGT, IGT and T2D P. obesus. These clones were sequenced to identify 51 genes, of which only 27 were previously known to encode secreted proteins. Three candidate genes not previously associated with obesity or type 2 diabetes, sushi domain containing 2, collagen and calcium-binding EGF domains 1 and periostin (Postn), as well as one gene known to be associated, complement component 1, were shown by RT-PCR to be differentially expressed in  skeletal muscle of P. obesus. Further characterization of the secreted protein Postn revealed it to be predominantly expressed in adipose tissue, with higher expression in visceral compared with subcutaneous adipose depots.

Conclusion: SST in conjunction with cDNA microarray technology is a powerful tool to identify differentially expressed secreted proteins involved in complex diseases such as obesity and type 2 diabetes. Furthermore, a number of candidate genes were identified, in particular, Postn, which may have a role in the development of obesity and type 2 diabetes.
Notes International Journal of Obesity advance online publication, 28 July 2009; doi:10.1038/ijo.2009.148
Language eng
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 920104 Diabetes
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2009
Copyright notice ©2009, Macmillan Publishers Limited
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019851

Document type: Journal Article
Collection: School of Medicine
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Created: Wed, 23 Sep 2009, 14:57:12 EST by Sally Morrigan

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