Differential calcineurin signalling activity and regeneration efficacy in diaphragm and limb muscles of dystrophic mdx mice

Stupka, Nicole, Michell, Belinda J., Kemp, Bruce E. and Lynch, Gordon S. 2006, Differential calcineurin signalling activity and regeneration efficacy in diaphragm and limb muscles of dystrophic mdx mice, Neuromuscular disorders, vol. 16, no. 5, pp. 337-346.

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Title Differential calcineurin signalling activity and regeneration efficacy in diaphragm and limb muscles of dystrophic mdx mice
Formatted title Differential calcineurin signalling activity and regeneration efficacy in diaphragm and limb muscles of dystrophic mdx mice
Author(s) Stupka, Nicole
Michell, Belinda J.
Kemp, Bruce E.
Lynch, Gordon S.
Journal name Neuromuscular disorders
Volume number 16
Issue number 5
Start page 337
End page 346
Publisher Elsevier B.V.
Place of publication Amsterdam, The Netherlands
Publication date 2006-05
ISSN 0960-8966
1873-2364
Keyword(s) calcineurin
NFATc1
myogenin
muscular dystrophy
diaphragm
skeletal muscle
muscle regeneration
Summary Calcineurin activity is essential for successful skeletal muscle regeneration in young mdx mice and in wild type mice following myotoxic injury and cryodamage. In mature myofibres of adult mdx mice, calcineurin stimulation can ameliorate the dystrophic pathology. The aim of this study was to test the hypothesis that the more severe dystrophic pathology of the diaphragm compared with hindlimb muscles of mdx mice could be attributed to aberrant calcineurin signalling and that due to ongoing regeneration calcineurin activity would be greater in muscles of adult mdx than wild type mice. Differences in markers of regeneration between tibialis anterior and diaphragm muscles were also characterised, to determine whether there was an association between regeneration efficacy and calcineurin activity in dystrophic muscles. In diaphragm muscles of adult mdx mice, the proportion of centrally nucleated fibres and developmental myosin heavy chain protein expression was lower and myogenin protein expression was higher than in tibialis anterior muscles. Calcineurin and activated NFATc1 protein content and calcineurin phosphatase activity were higher in muscles from mdx than wild type mice and calcineurin activation was greater in diaphragm than tibialis anterior muscles of mdx mice. Thus, despite greater calcineurin activity in diaphragm compared to hindlimb muscles, regeneration events downstream of myoblast differentiation and mediated by the injured myofibre were severely compromised.
Language eng
Field of Research 060110 Receptors and Membrane Biology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2006, Elsevier B.V.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019977

Document type: Journal Article
Collection: Institute for Technology Research and Innovation
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Created: Mon, 28 Sep 2009, 17:27:03 EST by Nicole Stupka

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