ß2-Agonist administration increases sarcoplasmic reticulum Ca2+-ATPase activity in aged rat skeletal muscle

Schertzer, Jonathon D., Ryall, James G., Plant, David R., Beitzel, Felice, Stupka, Nicole and Lynch, Gordon S. 2005, ß2-Agonist administration increases sarcoplasmic reticulum Ca2+-ATPase activity in aged rat skeletal muscle, American journal of physiology - endocrinology and metabolism, vol. 288, no. 3, pp. E526-E533.

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Title ß2-Agonist administration increases sarcoplasmic reticulum Ca2+-ATPase activity in aged rat skeletal muscle
Formatted title ß2-Agonist administration increases sarcoplasmic reticulum Ca2+-ATPase activity in aged rat skeletal muscle
Author(s) Schertzer, Jonathon D.
Ryall, James G.
Plant, David R.
Beitzel, Felice
Stupka, Nicole
Lynch, Gordon S.
Journal name American journal of physiology - endocrinology and metabolism
Volume number 288
Issue number 3
Start page E526
End page E533
Publisher American Physiological Society
Place of publication [Bethesda, Md.]
Publication date 2005-03
ISSN 0193-1849
1522-1555
Keyword(s) aging
calcium
beta-adrenoceptor
contractility
Summary Aging is associated with a slowing of skeletal muscle contractile properties, including a decreased rate of relaxation. In rats, the age-related decrease in the maximal rate of relaxation is reversed after 4-wk administration with the β2-adrenoceptor agonist (β2-agonist) fenoterol. Given the critical role of the sarcoplasmic reticulum (SR) in regulating intracellular Ca2+ transients and ultimately the time course of muscle contraction and relaxation, we tested the hypothesis that the mechanisms of action of fenoterol are mediated by alterations in SR proteins. Sarcoendoplasmic reticulum Ca2+-ATPase (SERCA) kinetic properties were assessed in muscle homogenates and enriched SR membranes isolated from the red (RG) and white (WG) portions of the gastrocnemius muscle in adult (16 mo) and aged (28 mo) F344 rats that had been administered fenoterol for 4 wk (1.4 mg/kg/day ip, in saline) or vehicle only. Aging was associated with a 29% decrease in the maximal activity (Vmax) of SERCA in the RG but not in the WG muscles. Fenoterol treatment increased the Vmax of SERCA and SERCA1 protein levels in RG and WG. In the RG, fenoterol administration reversed an age-related selective nitration of the SERCA2a isoform. Our findings demonstrate that the mechanisms underlying age-related changes in contractile properties are fiber type dependent, whereas the effects of fenoterol administration are independent of age and fiber type.
Language eng
Field of Research 060110 Receptors and Membrane Biology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2005, American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019979

Document type: Journal Article
Collection: Institute for Technology Research and Innovation
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Created: Mon, 28 Sep 2009, 17:42:47 EST by Nicole Stupka

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