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Changes in contractile activation characteristics of rat fast and slow skeletal muscle fibres during regeneration

Gregorevic, Paul, Plant, David R., Stupka, Nicole and Lynch, Gordon S. 2004, Changes in contractile activation characteristics of rat fast and slow skeletal muscle fibres during regeneration, Journal of physiology, vol. 558, no. 2, pp. 549-560.

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Title Changes in contractile activation characteristics of rat fast and slow skeletal muscle fibres during regeneration
Author(s) Gregorevic, Paul
Plant, David R.
Stupka, Nicole
Lynch, Gordon S.
Journal name Journal of physiology
Volume number 558
Issue number 2
Start page 549
End page 560
Publisher Cambridge University Press
Place of publication London, England
Publication date 2004-07
ISSN 0022-3751
Summary Damaged skeletal muscle fibres are replaced with new contractile units via muscle regeneration. Regenerating muscle fibres synthesize functionally distinct isoforms of contractile and regulatory proteins but little is known of their functional properties during the regeneration process. An advantage of utilizing single muscle fibre preparations is that assessment of their function is based on the overall characteristics of the contractile apparatus and regulatory system and as such, these preparations are sensitive in revealing not only coarse, but also subtle functional differences between muscle fibres. We examined the Ca2+- and Sr2+-activated contractile characteristics of permeabilized fibres from rat fast-twitch (extensor digitorum longus) and slow-twitch (soleus) muscles at 7, 14 and 21 days following myotoxic injury, to test the hypothesis that fibres from regenerating fast and slow muscles have different functional characteristics to fibres from uninjured muscles. Regenerating muscle fibres had ∼10% of the maximal force producing capacity (Po) of control (uninjured) fibres, and an altered sensitivity to Ca2+ and Sr2+ at 7 days post-injury. Increased force production and a shift in Ca2+ sensitivity consistent with fibre maturation were observed during regeneration such that Po was restored to 36–45% of that in control fibres by 21 days, and sensitivity to Ca2+ and Sr2+ was similar to that of control (uninjured) fibres. The findings support the hypothesis that regenerating muscle fibres have different contractile activation characteristics compared with mature fibres, and that they adopt properties of mature fast- or slow-twitch muscle fibres in a progressive manner as the regeneration process is completed.
Language eng
Field of Research 060110 Receptors and Membrane Biology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2004, Cambridge University Press
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019980

Document type: Journal Article
Collections: Institute for Technology Research and Innovation
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Created: Mon, 28 Sep 2009, 17:46:10 EST by Nicole Stupka