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Cellular adaption to repeated eccentric exercise-induced muscle damage

Stupka, Nicole, Tarnopolsky, Mark A., Yardley, Nick and Phillips, Stuart M. 2001, Cellular adaption to repeated eccentric exercise-induced muscle damage, Journal of applied physiology, vol. 91, no. 4, pp. 1669-1678.

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Title Cellular adaption to repeated eccentric exercise-induced muscle damage
Author(s) Stupka, Nicole
Tarnopolsky, Mark A.
Yardley, Nick
Phillips, Stuart M.
Journal name Journal of applied physiology
Volume number 91
Issue number 4
Start page 1669
End page 1678
Publisher American Physiological Society
Place of publication [Bethesda, Md.]
Publication date 2001-10
ISSN 8750-7587
1522-1601
Keyword(s) z-disk streaming
inflammatory cells
proteolysis
gender-based difference
Summary Eccentrically biased exercise results in skeletal muscle damage and stimulates adaptations in muscle, whereby indexes of damage are attenuated when the exercise is repeated. We hypothesized that changes in ultrastructural damage, inflammatory cell infiltration, and markers of proteolysis in skeletal muscle would come about as a result of repeated eccentric exercise and that gender may affect this adaptive response. Untrained male (n = 8) and female (n = 8) subjects performed two bouts (bout 1 and bout 2), separated by 5.5 wk, of 36 repetitions of unilateral, eccentric leg press and 100 repetitions of unilateral, eccentric knee extension exercises (at 120% of their concentric single repetition maximum), the subjects' contralateral nonexercised leg served as a control (rest). Biopsies were taken from the vastus lateralis from each leg 24 h postexercise. After bout 2, the postexercise force deficit and the rise in serum creatine kinase (CK) activity were attenuated. Women had lower serum CK activity compared with men at all times (P < 0.05), but there were no gender differences in the relative magnitude of the force deficit. Muscle Z-disk streaming, quantified by using light microscopy, was elevated vs. rest only after bout 1 (P < 0.05), with no gender difference. Muscle neutrophil counts were significantly greater in women 24 h after bout 2 vs. rest and bout 1 (P < 0.05) but were unchanged in men. Muscle macrophages were elevated in men and women after bout 1 andbout 2 (P < 0.05). Muscle protein content of the regulatory calpain subunit remained unchanged whereas ubiquitin-conjugated protein content was increased after both bouts (P < 0.05), with a greater increase after bout 2. We conclude that adaptations to eccentric exercise are associated with attenuated serum CK activity and, potentially, an increase in the activity of the ubiquitin proteosome proteolytic pathway.
Language eng
Field of Research 060110 Receptors and Membrane Biology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2001, the American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019983

Document type: Journal Article
Collection: Institute for Technology Research and Innovation
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Created: Mon, 28 Sep 2009, 17:57:16 EST by Nicole Stupka