Effects of ovariectomy and estrogen on ischemia-reperfusion injury in hindlimbs of female rats

Stupka, Nicole and Tiidus, Peter M. 2001, Effects of ovariectomy and estrogen on ischemia-reperfusion injury in hindlimbs of female rats, Journal of applied physiology, vol. 91, no. 4, pp. 1828-1835.

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Title Effects of ovariectomy and estrogen on ischemia-reperfusion injury in hindlimbs of female rats
Author(s) Stupka, NicoleORCID iD for Stupka, Nicole orcid.org/0000-0002-1000-1707
Tiidus, Peter M.
Journal name Journal of applied physiology
Volume number 91
Issue number 4
Start page 1828
End page 1835
Publisher American Physiological Society
Place of publication [Bethesda, Md.]
Publication date 2001-10
ISSN 8750-7587
Keyword(s) inflammatory cells
creatine kinase
skeletal muscle
Summary The effects of estrogen and ovariectomy on indexes of muscle damage after 2 h of complete hindlimb ischemia and 2 h of reperfusion were investigated in female Sprague-Dawley rats. The rats were assigned to one of three experimental groups: ovariectomized with a 17-estradiol pellet implant (OE), ovariectomized with a placebo pellet implant (OP), or control with intact ovaries (R). It was hypothesized that following ischemia-reperfusion (I/R), muscle damage indexes [serum creatine kinase (CK) activity, calpain-like activity, inflammatory cell infiltration, and markers of lipid peroxidation (thiobarbituric-reactive substances)] would be lower in the OE and R rats compared with the OP rats due to the protective effects of estrogen. Serum CK activity following I/R was greater (P < 0.01) in the R rats vs. OP rats and similar in the OP and OE rats. Calpain-like activity was greatest in the R rats (P < 0.01) and similar in the OP and OE rats. Neutrophil infiltration was assessed using the myeloperoxidase (MPO) assay and immunohistochemical staining for CD43-positive (CD43+) cells. MPO activity was lower (P < 0.05) in the OE rats compared with any other group and similar in the OP and R rats. The number of CD43+ cells was greater (P < 0.01) in the OP rats compared with the OE and R rats and similar in the OE and R rats. The OE rats had lower (P < 0.05) thiobarbituric-reactive substance content following I/R compared with the R and OP rats. Indexes of muscle damage were consistently attenuated in the OE rats but not in the R rats. A 10-fold difference in serum estrogen content may mediate this. Surprisingly, serum CK activity and muscle calpain-like activity were lower (P < 0.05) in the OP rats compared with the R rats. Increases in serum insulin-like growth factor-1 content (P < 0.05) due to ovariectomy were hypothesized to account for this finding. Thus both ovariectomy and estrogen supplementation have differential effects on indexes of I/R muscle damage.
Language eng
Field of Research 060110 Receptors and Membrane Biology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2001, American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019984

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Created: Mon, 28 Sep 2009, 17:59:46 EST by Nicole Stupka

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