Effects of ovariectomy and estrogen on ischemia-reperfusion injury in hindlimbs of female rats

Stupka, Nicole and Tiidus, Peter M. 2001, Effects of ovariectomy and estrogen on ischemia-reperfusion injury in hindlimbs of female rats, Journal of applied physiology, vol. 91, no. 4, pp. 1828-1835.

Attached Files
Name Description MIMEType Size Downloads

Title Effects of ovariectomy and estrogen on ischemia-reperfusion injury in hindlimbs of female rats
Author(s) Stupka, Nicole
Tiidus, Peter M.
Journal name Journal of applied physiology
Volume number 91
Issue number 4
Start page 1828
End page 1835
Publisher American Physiological Society
Place of publication [Bethesda, Md.]
Publication date 2001-10
ISSN 8750-7587
1522-1601
Keyword(s) inflammatory cells
myeloperoxidase
calpain
creatine kinase
skeletal muscle
Summary The effects of estrogen and ovariectomy on indexes of muscle damage after 2 h of complete hindlimb ischemia and 2 h of reperfusion were investigated in female Sprague-Dawley rats. The rats were assigned to one of three experimental groups: ovariectomized with a 17-estradiol pellet implant (OE), ovariectomized with a placebo pellet implant (OP), or control with intact ovaries (R). It was hypothesized that following ischemia-reperfusion (I/R), muscle damage indexes [serum creatine kinase (CK) activity, calpain-like activity, inflammatory cell infiltration, and markers of lipid peroxidation (thiobarbituric-reactive substances)] would be lower in the OE and R rats compared with the OP rats due to the protective effects of estrogen. Serum CK activity following I/R was greater (P < 0.01) in the R rats vs. OP rats and similar in the OP and OE rats. Calpain-like activity was greatest in the R rats (P < 0.01) and similar in the OP and OE rats. Neutrophil infiltration was assessed using the myeloperoxidase (MPO) assay and immunohistochemical staining for CD43-positive (CD43+) cells. MPO activity was lower (P < 0.05) in the OE rats compared with any other group and similar in the OP and R rats. The number of CD43+ cells was greater (P < 0.01) in the OP rats compared with the OE and R rats and similar in the OE and R rats. The OE rats had lower (P < 0.05) thiobarbituric-reactive substance content following I/R compared with the R and OP rats. Indexes of muscle damage were consistently attenuated in the OE rats but not in the R rats. A 10-fold difference in serum estrogen content may mediate this. Surprisingly, serum CK activity and muscle calpain-like activity were lower (P < 0.05) in the OP rats compared with the R rats. Increases in serum insulin-like growth factor-1 content (P < 0.05) due to ovariectomy were hypothesized to account for this finding. Thus both ovariectomy and estrogen supplementation have differential effects on indexes of I/R muscle damage.
Language eng
Field of Research 060110 Receptors and Membrane Biology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2001, American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019984

Document type: Journal Article
Collection: Institute for Technology Research and Innovation
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 42 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 334 Abstract Views, 1 File Downloads  -  Detailed Statistics
Created: Mon, 28 Sep 2009, 17:59:46 EST by Nicole Stupka

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.