The measurement of GLUT4 translocation in 3T3-L1 adipocytes

Konstantopoulos, Nicky and Molero, Juan Carlos 2009, The measurement of GLUT4 translocation in 3T3-L1 adipocytes. In Stocker, Claire (ed), Type 2 diabetes: methods and protocols, Humana Press, Totowa, N.J., pp.111-135, doi: 10.1007/978-1-59745-448-3_10.

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Title The measurement of GLUT4 translocation in 3T3-L1 adipocytes
Author(s) Konstantopoulos, Nicky
Molero, Juan Carlos
Title of book Type 2 diabetes: methods and protocols
Editor(s) Stocker, Claire
Publication date 2009
Series Methods in molecular biology ; 560
Chapter number 10
Total chapters 17
Start page 111
End page 135
Total pages 25
Publisher Humana Press
Place of Publication Totowa, N.J.
Keyword(s) GLUT4
translocation assay
plasma membrane lawns
subcellular fractionation
Summary Type 2 diabetes (T2D) is one of the fastest growing threats to human health in westernised and developing countries and is associated with central obesity, atherosclerosis, dyslipidaemia, hyperinsulinaemia and  hypertension. Insulin resistance, defined as a diminished response to ordinary levels of circulating insulin in one or more peripheral tissues, is an integral feature of T2D pathophysiology. This includes an impairment of insulin to inhibit hepatic glucose output and to stimulate glucose disposal into muscle and fat. While insulin is responsible for a number of specific biological responses, stimulation of glucose transport is critical for the maintenance of glucose homeostasis. The primary mechanism for insulin stimulation of glucose uptake into muscle and fat is the translocation of glucose transporter 4 (GLUT4) to the cell surface from intracellular storage vesicles within the cell. A major advantage in focussing on insulin regulation of glucose transport is that this represents the endpoint of multiple upstream signalling pathways. This chapter describes the measurement of GLUT4 translocation in cultured cells and its potential application for both  mechanistic and therapeutic studies.
ISBN 1934115150
Language eng
DOI 10.1007/978-1-59745-448-3_10
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category B1 Book chapter
ERA Research output type B Book chapter
HERDC collection year 2009
Copyright notice ©2009, Humana Press
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