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Preliminary evaluation of monolithic column high-performance liquid chromatography with tris(2,2’-bipyridyl)ruthenium(II) chemiluminescence detection for the determination of quetiapine in human body fluids Bellomarino, Sara A., Brown, Allyson J., Conlan, Xavier A. and Barnett, Neil W 2009, Preliminary evaluation of monolithic column high-performance liquid chromatography with tris(2,2’-bipyridyl)ruthenium(II) chemiluminescence detection for the determination of quetiapine in human body fluids, Talanta, vol. 77, no. 5, pp. 1873-1876. Attached Files Name Description MIMEType Size Downloads Title Preliminary evaluation of monolithic column high-performance liquid chromatography with tris(2,2’-bipyridyl)ruthenium(II) chemiluminescence detection for the determination of quetiapine in human body fluids Author(s) Bellomarino, Sara A. Brown, Allyson J. Conlan, Xavier A. Barnett, Neil W Journal name Talanta Volume number 77 Issue number 5 Start page 1873 End page 1876 Publisher Elsevier B.V. Place of publication Amsterdam, The Netherlands Publication date 2009-03-15 ISSN 0039-9140 1873-3573 Keyword(s) Quetiapine Metabolites Serum Urine Tris(2,2-bipyridyl)ruthenium(II) chemiluminescence Monolithic HPLC Summary High-performance liquid chromatography (HPLC) with tris(2,2-bipyridyl)ruthenium(II) chemiluminescence detection methodology is reported for the determination of the atypical antipsychotic drug quetiapine and the observation of its major active and inactive metabolites in human urine and serum. The method uses a monolithic chromatographic column allowing high flow rates of 3mL min−1 enabling rapid quantification. Flow injection analysis (FIA) with tris(2,2-bipyridyl)ruthenium(II) chemiluminescence detection and HPLC time of flight mass spectrometry (TOF-MS) were used for the determination of quetiapine in a pharmaceutical preparation to establish its suitability as a calibration standard. The limit of detection achieved with FIA was 2×10−11 mol L−1 in simple aqueous solution. The limits of detection achieved with HPLC were 7×10−8 and 2×10−10 mol L−1 in urine and serum, respectively. The calibration range for FIA was between 5×10−9 and 1×10−6 mol L−1. The calibration ranges for HPLC were between 1×10−7–1×10−4 and 1×10−8–1×10−4 mol L−1 in urine and serum, respectively. The quetiapine concentrations in patient samples were found to be 3×10−6 mol L−1 in urine and 7×10−7 mol L−1 in serum. Without the need for preconcentration, the HPLC detection limits compared favourably with those in previously published methodologies. The metabolites were identified using HPLC-TOF-MS. Language eng Field of Research 030108 Separation Science Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2008, Elsevier B.V. Persistent URL http://hdl.handle.net/10536/DRO/DU:30021102 Document type: Journal Article Collection: School of Life and Environmental Sciences Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Wed, 25 Nov 2009, 11:09:42 EST Wed, 25 Nov 2009, 11:12:23 EST Thu, 26 Nov 2009, 08:49:29 EST Thu, 26 Nov 2009, 08:51:36 EST Tue, 12 Jan 2010, 13:32:45 EST Tue, 12 Jan 2010, 13:46:15 EST Mon, 15 Mar 2010, 12:10:58 EST Thu, 01 Jul 2010, 17:08:39 EST Thu, 03 Oct 2013, 10:40:02 EST Filtered Full Citation counts:   Cited 14 times in TR Web of Science Cited 21 times in Scopus Search Google Scholar Access Statistics: 331 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Wed, 25 Nov 2009, 11:09:41 EST by Teresa Treffry

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