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Characterization of microsatellite DNA markers for a mahseer species, Tor tambroides (Cyprinidae) and cross-amplification in four congeners

Nguyen, Thuy, Baranski, Matthew, Rourke, Meaghan and McPartlan, Helen 2006, Characterization of microsatellite DNA markers for a mahseer species, Tor tambroides (Cyprinidae) and cross-amplification in four congeners, Molecular ecology notes, vol. 7, no. 1, pp. 109-112, doi: 10.1111/j.1471-8286.2006.01546.x.

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Title Characterization of microsatellite DNA markers for a mahseer species, Tor tambroides (Cyprinidae) and cross-amplification in four congeners
Formatted title Characterization of microsatellite DNA markers for a mahseer species, Tor tambroides (Cyprinidae) and cross-amplification in four congeners
Author(s) Nguyen, Thuy
Baranski, Matthew
Rourke, Meaghan
McPartlan, Helen
Journal name Molecular ecology notes
Volume number 7
Issue number 1
Start page 109
End page 112
Publisher Blackwell Science
Place of publication Oxford, England
Publication date 2006-01
ISSN 1471-8278
1755-0998
Keyword(s) aquaculture
mahseer
management
microsatellites
Tor tambroides
Summary This study reports the isolation and characterization of microsatellite DNA markers in a mahseer species, Tor tambroides (Pisces, Cyprinidae). Of a total of 14 loci evaluated, 10 were polymorphic in T. tambroides samples, with an average of 2.86 alleles per locus. Deviations from Hardy–Weinberg equilibrium were observed at one locus and there was no indication of linkage disequilibrium among loci. A high level of cross-amplification among four congeners was achieved, with 12 loci successfully amplifying and 11 loci showing polymorphism in at least one other species. These markers will be a useful resource for population genetic studies and broodstock management of closely related mahseer species.
Language eng
DOI 10.1111/j.1471-8286.2006.01546.x
Field of Research 070401 Aquaculture
HERDC Research category C4.1 Letter or note
Copyright notice ©2007, The Authors
Persistent URL http://hdl.handle.net/10536/DRO/DU:30021374

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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Created: Mon, 14 Dec 2009, 21:18:09 EST by Thuy Nguyen

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