Effect of carbohydrate ingestion on exercise-induced alterations in metabolic gene expression

Cluberton, Laura, McGee, Sean, Murphy, Robyn and Hargreaves, Mark 2005, Effect of carbohydrate ingestion on exercise-induced alterations in metabolic gene expression, Journal of applied physiology, vol. 99, no. 4, pp. 1359-1363.

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Title Effect of carbohydrate ingestion on exercise-induced alterations in metabolic gene expression
Author(s) Cluberton, Laura
McGee, Sean
Murphy, Robyn
Hargreaves, Mark
Journal name Journal of applied physiology
Volume number 99
Issue number 4
Start page 1359
End page 1363
Publisher American Physiological Society
Place of publication [Bethesda, Md.]
Publication date 2005-10
ISSN 8750-7587
1522-1601
Keyword(s) transcription
skeletal muscle
Summary Skeletal muscle possesses a high degree of plasticity and can adapt to both the physical and metabolic challenges that it faces. An acute bout of exercise is sufficient to induce the expression of a variety of metabolic genes, such as GLUT4, pyruvate dehydrogenase kinase 4 (PDK-4), uncoupling protein-3 (UCP3), and peroxisome proliferator-activated receptor-? coactivator 1 (PGC-1). Reducing muscle glycogen levels before exercise potentiates the effect of exercise on many genes. Similarly, altered substrate availability induces transcription of many of these genes. The purpose of this study was to determine whether glucose ingestion attenuates the exercise-induced increase in a variety of exercise-responsive genes. Six male subjects (28 ± 7 yr; 83 ± 3 kg; peak pulmonary oxygen uptake = 46 ± 6 ml·kg–1·min–1) performed 60 min of cycling at 74 ± 2% of peak pulmonary oxygen uptake on two separate occasions. On one occasion, subjects ingested a 6% carbohydrate drink. On the other occasion, subjects ingested an equal volume of a sweet placebo. Muscle samples were obtained from vastus lateralis at rest, immediately after exercise, and 3 h after exercise. PDK-4, UCP3, PGC-1, and GLUT4 mRNA levels were measured on these samples using real-time RT-PCR. Glucose ingestion attenuated (P < 0.05) the exercise-induced increase in PDK-4 and UCP3 mRNA. A similar trend (P = 0.09) was observed for GLUT4 mRNA. In contrast, PGC-1 mRNA increased following exercise to the same extent in both conditions. These data suggest that glucose availability can modulate the effect of exercise on metabolic gene expression.
Language eng
Field of Research 060405 Gene Expression (incl Microarray and other genome-wide approaches)
060603 Animal Physiology - Systems
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2005, American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30021454

Document type: Journal Article
Collection: School of Medicine
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