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Co-localization and distribution of corticotrophin-releasing hormone, arginine vasopressin and enkephalin in the paraventricular nucleus of sheep : a sex comparison

Rivalland, E.T.A., Iqbal, J., Turner, Anne I., Clarke, I. J. and Tilbrook, A . J. 2005, Co-localization and distribution of corticotrophin-releasing hormone, arginine vasopressin and enkephalin in the paraventricular nucleus of sheep : a sex comparison, Neuroscience, vol. 132, no. 3, pp. 755-766, doi: 10.1016/j.neuroscience.2005.01.045.

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Title Co-localization and distribution of corticotrophin-releasing hormone, arginine vasopressin and enkephalin in the paraventricular nucleus of sheep : a sex comparison
Author(s) Rivalland, E.T.A.
Iqbal, J.
Turner, Anne I.ORCID iD for Turner, Anne I. orcid.org/0000-0002-0682-2860
Clarke, I. J.
Tilbrook, A . J.
Journal name Neuroscience
Volume number 132
Issue number 3
Start page 755
End page 766
Publisher Elsevier Ltd.
Place of publication Amsterdam, Netherlands
Publication date 2005
ISSN 0306-4522
1873-7544
Keyword(s) hypothalamo-pituitary-adrenal axis
immunohistochemistry
gonadectomy
hypothalamus
neuropeptides
Summary The paraventricular nucleus (PVN) is integral to regulation of the hypothalamo-pituitary-adrenal (HPA) axis and contains cells producing corticotrophin-releasing hormone (CRH), arginine vasopressin (AVP) and enkephalin. We used immunohistochemistry to map these peptides and to resolve the extent of co-localization within PVN cells in intact and gonadectomized male and female sheep. Immunoreactive (ir) CRH, AVP and enkephalin cells were mapped in two rams and two ewes at 180 μm intervals throughout the rostro-caudal extent of the PVN. Similar distributions of AVP-ir cells occurred in both sexes whereas CRH-ir and enkephalin-ir cells extended more rostrally in rams. In groups (n=4) of intact and gonadectomized sheep of both sexes, co-localization and distribution of neuropeptides was influenced by sex and gonadectomy. Males had more AVP and CRH cells than females. Intact animals had more AVP cells than gonadectomized animals. There were no differences between groups in the number or percentage of cells that stained for both CRH and AVP or in the number of cells that stained for both CRH and enkephalin. Differences were observed in the percentage of enkephalin cells that contained CRH with males having a greater percentage of co-localized cells than did females. Differences were also observed in the number and percentage of cells that stained for both enkephalin and AVP; the number of cells that stained for both neuropeptides was greater in males than in females and greater in intact animals than in gonadectomized animals. Differences were observed in the percentage of AVP cells that contained enkephalin, and in the percentage of enkephalin cells that contained AVP with males having a greater percentage of co-localized cells than did females. We conclude that sex and gonadal status affect peptide distribution in the PVN of the sheep which may provide an anatomical basis for sex differences in HPA axis
Language eng
DOI 10.1016/j.neuroscience.2005.01.045
Field of Research 060105 Cell Neurochemistry
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2005 IBRO Published by Elsevier Ltd
Persistent URL http://hdl.handle.net/10536/DRO/DU:30022059

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