Histological assessment of intermediate - and long-term creatine monohydrate supplementation in mice and rats

Tarnopolsky, M. A., Bourgeois, J. M., Snow, R., Keys, S., Roy, B. D., Kwiecien, J. M. and Turnbull, J. 2003, Histological assessment of intermediate - and long-term creatine monohydrate supplementation in mice and rats, American journal of physiology : regulatory, integrative and comparative physiology, vol. 285, no. 4, pp. R762-R769.


Title Histological assessment of intermediate - and long-term creatine monohydrate supplementation in mice and rats
Author(s) Tarnopolsky, M. A.
Bourgeois, J. M.
Snow, R.
Keys, S.
Roy, B. D.
Kwiecien, J. M.
Turnbull, J.
Journal name American journal of physiology : regulatory, integrative and comparative physiology
Volume number 285
Issue number 4
Start page R762
End page R769
Publisher American Psychological Society
Place of publication Bethesda, Md.
Publication date 2003-10
ISSN 0363-6119
1522-1490
Keyword(s) dietary supplements
hepatitis
drug toxicity
side effects
Summary Creatine monohydrate (CrM) supplementation appears to be relatively safe based on data from short-term and intermediate-term human studies and results from several therapeutic trials. The purpose of the current study was to characterize pathological changes after intermediate-term and long-term CrM supplementation in mice [healthy control and SOD1 (G93A) transgenic] and rats (prednisolone and nonprednisolone treated). Histological assessment (18-20 organs/tissues) was performed on G93A mice after 159 days, and in Sprague-Dawley rats after 365 days, of CrM supplementation (2% wt/wt) compared with control feed. Liver histology was also evaluated in CD-1 mice after 300 days of low-dose CrM supplementation (0.025 and 0.05 g · kg-1 · day-1) and in Sprague-Dawley rats after 52 days of CrM supplementation (2% wt/wt) with and without prednisolone. Areas of hepatitis were observed in the livers of the CrM-supplemented G93A mice (P < 0.05), with no significant inflammatory lesions in any of the other 18-20 tissues/organs that were evaluated. The CD-1 mice also showed significant hepatic inflammatory lesions (P < 0.05), yet there was no negative effect of CrM on liver histology in the Sprague-Dawley rats after intermediate-term or long-term supplementation nor was inflammation seen in any other tissues/organs (P = not significant). Dietary CrM supplementation can induce inflammatory changes in the liver of mice, but not rats. The observed inflammatory changes in the murine liver must be considered in the evaluation of hepatic metabolism in CrM-supplemented mice. Species differences must be considered in the evaluation of toxicological and physiological studies.
Language eng
Field of Research 060604 Comparative Physiology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2003, American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30022476

Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 27 times in TR Web of Science
Scopus Citation Count Cited 29 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 369 Abstract Views  -  Detailed Statistics
Created: Tue, 19 Jan 2010, 13:52:21 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.