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Bioinformatic and expression analyses of genes mediating zinc homeostasis in Nostoc punctiforme

Hudek, Lee, Rai, L.C., Freestone, David, Michalczyk, Agnes, Gibson, Maria, Song, Y.F. and Ackland, M. Leigh 2009, Bioinformatic and expression analyses of genes mediating zinc homeostasis in Nostoc punctiforme, Applied and environmental microbiology, vol. 75, no. 3, pp. 784-791, doi: 10.1128/AEM.02481-08.

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Title Bioinformatic and expression analyses of genes mediating zinc homeostasis in Nostoc punctiforme
Formatted title Bioinformatic and expression analyses of genes mediating zinc homeostasis in Nostoc punctiforme
Author(s) Hudek, Lee
Rai, L.C.
Freestone, David
Michalczyk, AgnesORCID iD for Michalczyk, Agnes orcid.org/0000-0001-5716-0783
Gibson, Maria
Song, Y.F.
Ackland, M. LeighORCID iD for Ackland, M. Leigh orcid.org/0000-0002-7474-6556
Journal name Applied and environmental microbiology
Volume number 75
Issue number 3
Start page 784
End page 791
Total pages 8
Publisher American Society for Microbiology
Place of publication Washington, D.C.
Publication date 2009-02
ISSN 0099-2240
1098-5336
Summary Zinc homeostasis was investigated in Nostoc punctiforme. Cell tolerance to Zn2+ over 14 days showed that ZnCl2 levels above 22 µM significantly reduced cell viability. After 3 days in 22 µM ZnCl2, ca. 12% of the Zn2+ was in an EDTA-resistant component, suggesting an intracellular localization. Zinquin fluorescence was detected within cells exposed to concentrations up to 37 µM relative to 0 µM treatment. Radiolabeled 65Zn showed Zn2+ uptake increased over a 3-day period, while efflux occurred more rapidly within a 3-h time period. Four putative genes involved in Zn2+ uptake and efflux in N. punctiforme were identified: (i) the predicted Co/Zn/Cd cation transporter, putative CDF; (ii) the predicted divalent heavy-metal cation transporter, putative Zip; (iii) the ATPase component and Fe/Zn uptake regulation protein, putative Fur; and (iv) an ABC-type Mn/Zn transport system, putative zinc ZnuC, ZnuABC system component. Quantitative real-time PCR indicated the responsiveness of all four genes to 22 µM ZnCl2 within 3 h, followed by a reduction to below basal levels after 24 h by putative ZIP, ZnuC, and Fur and a reduction to below basal level after 72 h by putative CDF efflux gene. These results demonstrate differential regulation of zinc transporters over time, indicating a role for them in zinc homeostasis in N. punctiforme.
Language eng
DOI 10.1128/AEM.02481-08
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
HERDC collection year 2009
Copyright notice ©2009, American Society for Microbiology
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30022586

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.