Deakin home > Deakin University Library > Deakin Research Online > Developmental changes in the expression of creatine synthesizing enzymes and creatine transporter in a precocial rodent, the spiny mouse
Openly accessible

Developmental changes in the expression of creatine synthesizing enzymes and creatine transporter in a precocial rodent, the spiny mouse

Ireland, Zoe, Russell, Aaron P., Wallimann, Theo, Walker, David W. and Snow, Rod 2009, Developmental changes in the expression of creatine synthesizing enzymes and creatine transporter in a precocial rodent, the spiny mouse, BMC developmental biology, vol. 9, no. 39, pp. 1-12.

Attached Files (Some files may be inaccessible until you login with your DRO credentials)
Name Description MIMEType Size Downloads
russell-developmentalchanges-2009.pdf Published version application/pdf 851.32KB 66

Title Developmental changes in the expression of creatine synthesizing enzymes and creatine transporter in a precocial rodent, the spiny mouse
Author(s) Ireland, Zoe
Russell, Aaron P.
Wallimann, Theo
Walker, David W.
Snow, Rod
Journal name BMC developmental biology
Volume number 9
Issue number 39
Start page 1
End page 12
Total pages 12
Publisher BioMed Central
Place of publication London, England
Publication date 2009
ISSN 1471-213X
Summary Background : Creatine synthesis takes place predominately in the kidney and liver via a two-step process involving AGAT (L-arginine:glycine amidinotransferase) and GAMT (guanidinoacetate methyltransferase). Creatine is taken into cells via the creatine transporter (CrT), where it plays an essential role in energy homeostasis, particularly for tissues with high and fluctuating energy demands. Very little is known of the fetal requirement for creatine and how this may change with advancing pregnancy and into the early neonatal period. Using the spiny mouse as a model of human perinatal development, the purpose of the present study was to comprehensively examine the development of the creatine synthesis and transport systems.

Results : The estimated amount of total creatine in the placenta and brain significantly increased in the second half of pregnancy, coinciding with a significant increase in expression of CrT mRNA. In the fetal brain, mRNA expression of AGAT increased steadily across the second half of pregnancy, although GAMT mRNA expression was relatively low until 34 days gestation (term is 38–39 days). In the fetal kidney and liver, AGAT and GAMT mRNA and protein expression were also relatively low until 34–37 days gestation. Between mid-gestation and term, neither AGAT or GAMT mRNA or protein could be detected in the placenta.

Conclusion : Our results suggest that in the spiny mouse, a species where, like the human, considerable organogenesis occurs before birth, there appears to be a limited capacity for endogenous creatine synthesis until approximately 0.9 of pregnancy. This implies that a maternal source of creatine, transferred across the placenta, may be essential until the creatine synthesis and transport system matures in preparation for birth. If these results also apply to the human, premature birth may increase the risk of creatine deficiency.
Notes This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Language eng
Field of Research 111401 Foetal Development and Medicine
Socio Economic Objective 940111 Ethnicity, Multiculturalism and Migrant Development and Welfare
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2009, Ireland et al.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30022696

Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 22 times in TR Web of Science
Scopus Citation Count Cited 20 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 406 Abstract Views, 66 File Downloads  -  Detailed Statistics
Created: Fri, 22 Jan 2010, 10:14:12 EST by Sally Morrigan