Revisiting delta-6 desaturate regulation by C18 unsaturated fatty acids, depending on the nutritional status

Skrzypski, Jeremy, Bellenger, Sandrine, Bellenger, Jerome, Sinclair, Andrew, Poisson, Jean-Pierre, Tessier, Christian, Rialland, Mickael and Narce, Michel 2009, Revisiting delta-6 desaturate regulation by C18 unsaturated fatty acids, depending on the nutritional status, Biochimie, vol. 91, no. 11-12, pp. 1443-1449, doi: 10.1016/j.biochi.2009.08.001.

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Title Revisiting delta-6 desaturate regulation by C18 unsaturated fatty acids, depending on the nutritional status
Author(s) Skrzypski, Jeremy
Bellenger, Sandrine
Bellenger, Jerome
Sinclair, Andrew
Poisson, Jean-Pierre
Tessier, Christian
Rialland, Mickael
Narce, Michel
Journal name Biochimie
Volume number 91
Issue number 11-12
Start page 1443
End page 1449
Total pages 7
Publisher Elsevier Masson SAS
Place of publication [Paris, France]
Publication date 2009-11
ISSN 0300-9084
Keyword(s) delta-6 desaturation
dietary fatty acids
liver lipids
linoleic acid
alpha-linolenic acid
oleic acid
Summary Dietary polyunsaturated fatty acids (PUFA) play a key role in regulating delta-6 desaturase (D6D), the key enzyme for long-chain PUFA biosynthesis. Nevertheless, the extent of their effects on this enzyme remains controversial and difficult to assess. It has been generally admitted that C18 unsaturated fatty acids (UFAs) regulate negatively delta-6 desaturase (D6D). This inhibition has been evidenced in regard to a high glucose/fat free (HG/FF) diet used in reference. However, several nutritional investigations did not evidence any inhibition of desaturases when feeding fatty acids.

Because the choice of the basal diet appeared to be of primary importance in such experiments, our goal was to reconsider the specific role of dietary UFAs on D6D regulation, depending on nutritional conditions. For that, sixteen adult Wistar rats were fed purified linoleic acid, α-linolenic acid or oleic acid, included in one of two diets at 4% by weight: an HG/FF or a high starch base (HS) where the pure UFAs replaced a mixed vegetable oil. Our results showed first that D6D specific activity was significantly greater when measured in presence of an HG/FF than with an HS/4% vegetable oil diet. Secondly, we found that linoleic and alpha-linolenic acids added to HG/FF reduced the specific activity of D6D. In contrast, when pure UFAs were added to an HS base, D6D specific activities remained unchanged or increased. Concordant results were obtained on D6D mRNA expression.

Altogether, this study evidenced the importance of the nutritional status in D6D regulation by C18 UFAs: when used as control, HG/FF diet stimulates D6D compared with a standard control diet containing starch and 4% fats, leading to an overestimation of the D6D regulation by UFAs. Then, UFAs should be considered as repressors for unsaturated fatty acid biosynthesis only in very specific nutritional conditions.
Language eng
DOI 10.1016/j.biochi.2009.08.001
Field of Research 111102 Dietetics and Nutrigenomics
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2009, Elsevier Masson SAS
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Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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