Identification and characterization of genes conferring salt tolerance to Escherichia coli from pond water metagenome

Kapardar, Raj Kishor, Ranjan, Ravi, Grover, Amit, Puri, Munish and Sharma, Rakesh 2010, Identification and characterization of genes conferring salt tolerance to Escherichia coli from pond water metagenome, Bioresource technology, vol. 101, no. 11, pp. 3917-3924.

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Title Identification and characterization of genes conferring salt tolerance to Escherichia coli from pond water metagenome
Formatted title Identification and characterization of genes conferring salt tolerance to Escherichia coli from pond water metagenome
Author(s) Kapardar, Raj Kishor
Ranjan, Ravi
Grover, Amit
Puri, Munish
Sharma, Rakesh
Journal name Bioresource technology
Volume number 101
Issue number 11
Start page 3917
End page 3924
Total pages 8
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2010-06
ISSN 0960-8524
1873-2976
Keyword(s) metagenome
salt tolerance
Enoyl-CoA hydratase
general stress protein
Summary Metagenomics provides culture-independent access to gene pool of the whole microbial communities. To identify genes responsible for salt tolerance in unculturable bacteria, Escherichia coli clones were enriched with an ability to grow at inhibitory NaCl concentrations (750 mM) from a pond water metagenomic library. From two unique clones, genes encoding for proteins with similarity to a putative general stress protein (GspM) harbouring GsiB domain and a putative enoyl-CoA hydratase (EchM) were identified to be responsible for salt tolerance. The gspM was expressed by its native promoter whereas the echM was expressed from the lacZ promoter of the plasmid. EchM was overexpressed with a hexahistidyl tag. Purified EchM showed crotonyl-CoA hydratase activity. These genes have potential application in generating salt tolerant recombinant bacteria or transgenic plants.
Language eng
Field of Research 100205 Environmental Marine Biotechnology
HERDC Research category C1.1 Refereed article in a scholarly journal
HERDC collection year 2010
Copyright notice ©2010, Elsevier Ltd.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30023627

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Created: Wed, 10 Feb 2010, 14:06:52 EST by Munish Puri

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