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Methionine regulates copper/hydrogen peroxide oxidation products of Aβ

Ali, Feda E., Separovic, Frances, Barrow, Colin J., Cherny, Robert A., Fraser, Fiona, Bush, Ashely I., Masters, Colin L. and Barnham, Kevin J. 2005, Methionine regulates copper/hydrogen peroxide oxidation products of Aβ, Journal of peptide science, vol. 11, no. 6, pp. 353-360.

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Title Methionine regulates copper/hydrogen peroxide oxidation products of Aβ
Author(s) Ali, Feda E.
Separovic, Frances
Barrow, Colin J.
Cherny, Robert A.
Fraser, Fiona
Bush, Ashely I.
Masters, Colin L.
Barnham, Kevin J.
Journal name Journal of peptide science
Volume number 11
Issue number 6
Start page 353
End page 360
Total pages 8
Publisher John Wiley & Sons
Place of publication Bognor Regis, England
Publication date 2005
ISSN 1075-2617
Summary Metal-catalysed oxidation (MCO) may play a causative role in the pathogenesis of Alzheimer's disease (AD). Amyloid peptide (A), the major biomarker of AD, in the presence of copper ions reduces Cu2+ to Cu+ and catalyses the formation of H2O2 that subsequently induces radicals through Fenton chemistry. A is also subject to attack by free radicals, where the presence of Cu2+ in conjunction with H2O2 catalyses oxygenation, primarily at the methionine sulfur atom. This work investigates MCO of A, to gain further insight into the role of oxidative stress in AD. By combining a fluorescence assay with gel electrophoresis to monitor MCO reactions of A (1-28) in the presence and absence of methionine it was determined that methionine can both protect some residues against MCO and promote the oxidation of Tyr(10) specifically. Electrospray ionization mass spectrometric analysis of methionine MCO products indicated the formation of methionine sulfoxide, methionine sulfone and related hydroxylated products. Similar products could be formed from the oxidation of Met(35) of A and may relate to changes in properties of the peptide following MCO.
Language eng
Field of Research 110106 Medical Biochemistry: Proteins and Peptides (incl Medical Proteomics)
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2004, John Wiley & Sons
Persistent URL http://hdl.handle.net/10536/DRO/DU:30023801

Document type: Journal Article
Collections: School of Life and Environmental Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.