Isolation, characterization and structural determination of a unique type of arabinogalactan from an immunostimulatory extract of Chlorella pyrenoidosa

Suárez, Erick Reyes, Kralovec, Jaroslav A., Noseda, Miguel D., Ewart, H. Stephen, Barrow, Colin J., Lumsden, Michael D. and Grindley, T. Bruce 2005, Isolation, characterization and structural determination of a unique type of arabinogalactan from an immunostimulatory extract of Chlorella pyrenoidosa, Carbohydrate research, vol. 340, no. 8, pp. 1489-1498.

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Title Isolation, characterization and structural determination of a unique type of arabinogalactan from an immunostimulatory extract of Chlorella pyrenoidosa
Author(s) Suárez, Erick Reyes
Kralovec, Jaroslav A.
Noseda, Miguel D.
Ewart, H. Stephen
Barrow, Colin J.
Lumsden, Michael D.
Grindley, T. Bruce
Journal name Carbohydrate research
Volume number 340
Issue number 8
Start page 1489
End page 1498
Total pages 10
Publisher Pergamon
Place of publication Oxford, England
Publication date 2005-06
ISSN 0008-6215
1873-426X
Summary An arabinogalactan was isolated from a hot water extract of freeze-dried cells of the green microalga, Chlorella pyrenoidosa. This hot water extract is a proprietary immunomodulator, with the trademark Respondin™ (ONC-107). The arabinogalactan was recovered from the ethanol-soluble fraction of the supernatant resulting from a process that involved controlled ethanol precipitation followed by size exclusion chromatography on Sephadex G-100, then Cetavlon precipitation. Sugar analyses, GC–MS data for (S)-2-octyl glycosides, and 1D and 2D NMR experiments established unambiguously that the repeating unit was →2)-α-l-Araf-(1→3)-[α-l-Araf-(1→4)]-β-d-Galp-(1→. This structure does not fit into any of the known classes of arabinogalactans. SEC/MALS experiments gave a molecular mass for the arabinogalactan isolated as 47 ± 4 kDa but the original structure was probably larger.
Language eng
Field of Research 060101 Analytical Biochemistry
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2005, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30023811

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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