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Rapid bioassay-guided screening of toxic substances in vegetable oils that shorten the life of SHRSP rats

Ratnayake, Sunil and Lewandowski, Paul 2010, Rapid bioassay-guided screening of toxic substances in vegetable oils that shorten the life of SHRSP rats, Lipids in health and disease, vol. 9, no. 13, pp. 3-14.

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Title Rapid bioassay-guided screening of toxic substances in vegetable oils that shorten the life of SHRSP rats
Author(s) Ratnayake, Sunil
Lewandowski, Paul
Journal name Lipids in health and disease
Volume number 9
Issue number 13
Start page 3
End page 14
Publisher BioMed Central
Place of publication London, England
Publication date 2010-02-02
ISSN 1476-511X
Summary It has been consistently reported that vegetable oils including canola oil have a life shortening effect in Stroke-Prone Spontaneously Hypertensive Rats (SHRSP) and this toxic effect is not due to the fatty acid composition of the oil. Although it is possible that the phytosterol content or type of phytosterol present in vegetable oils may play some role in the life shortening effect observed in SHRSP rats this is still not completely resolved. Furthermore supercritical CO2 fractionation of canola oil with subsequent testing in SHRSP rats identified safe and toxic fractions however, the compounds responsible for life shortening effect were not characterised. The conventional approach to screen toxic substances in oils using rats takes more than six months and involves large number of animals. In this article we describe how rapid bioassay-guided screening could be used to identify toxic substances derived from vegetable oils and/or processed foods fortified with vegetable oils. The technique incorporates sequential fractionation of oils/processed foods and subsequent treatment of human cell lines that can be used in place of animal studies to determine cytotoxicity of the fractions with structural elucidation of compounds of interest determined via HPLC-MS and GC-MS. The rapid bioassay-guided screening proposed would require two weeks to test multiple fractions from oils, compared with six months if animal experiments were used to screen toxic effects. Fractionation of oil before bio-assay enhances the effectiveness of the detection of active compounds as fractionation increases the relative concentration of minor components.
Notes This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Language eng
Field of Research 111199 Nutrition and Dietetics not elsewhere classified
Socio Economic Objective 920411 Nutrition
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2010, Ratnayake and Lewandowski
Persistent URL http://hdl.handle.net/10536/DRO/DU:30023828

Document type: Journal Article
Collections: School of Medicine
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Created: Mon, 22 Feb 2010, 11:19:45 EST by Paul Lewandowski