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Rapid bioassay-guided screening of toxic substances in vegetable oils that shorten the life of SHRSP rats Ratnayake, Sunil and Lewandowski, Paul 2010, Rapid bioassay-guided screening of toxic substances in vegetable oils that shorten the life of SHRSP rats, Lipids in health and disease, vol. 9, no. 13, pp. 3-14. Attached Files Name Description MIMEType Size Downloads lewandowski-rapidbioassayguided-2010.pdf Published version application/pdf 225.48KB 33 Title Rapid bioassay-guided screening of toxic substances in vegetable oils that shorten the life of SHRSP rats Author(s) Ratnayake, Sunil Lewandowski, Paul Journal name Lipids in health and disease Volume number 9 Issue number 13 Start page 3 End page 14 Publisher BioMed Central Place of publication London, England Publication date 2010-02-02 ISSN 1476-511X Summary It has been consistently reported that vegetable oils including canola oil have a life shortening effect in Stroke-Prone Spontaneously Hypertensive Rats (SHRSP) and this toxic effect is not due to the fatty acid composition of the oil. Although it is possible that the phytosterol content or type of phytosterol present in vegetable oils may play some role in the life shortening effect observed in SHRSP rats this is still not completely resolved. Furthermore supercritical CO2 fractionation of canola oil with subsequent testing in SHRSP rats identified safe and toxic fractions however, the compounds responsible for life shortening effect were not characterised. The conventional approach to screen toxic substances in oils using rats takes more than six months and involves large number of animals. In this article we describe how rapid bioassay-guided screening could be used to identify toxic substances derived from vegetable oils and/or processed foods fortified with vegetable oils. The technique incorporates sequential fractionation of oils/processed foods and subsequent treatment of human cell lines that can be used in place of animal studies to determine cytotoxicity of the fractions with structural elucidation of compounds of interest determined via HPLC-MS and GC-MS. The rapid bioassay-guided screening proposed would require two weeks to test multiple fractions from oils, compared with six months if animal experiments were used to screen toxic effects. Fractionation of oil before bio-assay enhances the effectiveness of the detection of active compounds as fractionation increases the relative concentration of minor components. Notes This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Language eng Field of Research 111199 Nutrition and Dietetics not elsewhere classified Socio Economic Objective 920411 Nutrition HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2010, Ratnayake and Lewandowski Persistent URL http://hdl.handle.net/10536/DRO/DU:30023828 Document type: Journal Article Collections: School of Medicine Open Access Collection Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Mon, 22 Feb 2010, 11:19:48 EST Mon, 22 Feb 2010, 15:09:01 EST Tue, 23 Feb 2010, 13:39:06 EST Tue, 23 Feb 2010, 13:40:30 EST Wed, 24 Feb 2010, 11:37:37 EST Thu, 25 Feb 2010, 09:25:26 EST Fri, 07 Jan 2011, 12:48:48 EST Thu, 16 Jun 2011, 12:22:28 EST Mon, 11 Jul 2011, 10:26:41 EST Tue, 12 Jul 2011, 09:18:20 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Search Google Scholar Access Statistics: 345 Abstract Views, 35 File Downloads  -  Detailed Statistics Created: Mon, 22 Feb 2010, 11:19:45 EST by Paul Lewandowski

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.