Threshold effects of glucose transporter-4 (GLUT4) deficiency on cardiac glucose uptake and development of hypertrophy

Kaczmarczyk, S. J., Andrikopoulos, S., Favaloro, J., Domenighetti, A. A., Dunn, A., Ernst, M., Grail, D., Fodero-Tavoletti, M., Huggins, C. E., Delbridge, L. M., Zajac, J. D. and Proietto, J. 2003, Threshold effects of glucose transporter-4 (GLUT4) deficiency on cardiac glucose uptake and development of hypertrophy, Journal of molecular endocrinology, vol. 31, no. 3, pp. 449-459.


Title Threshold effects of glucose transporter-4 (GLUT4) deficiency on cardiac glucose uptake and development of hypertrophy
Author(s) Kaczmarczyk, S. J.
Andrikopoulos, S.
Favaloro, J.
Domenighetti, A. A.
Dunn, A.
Ernst, M.
Grail, D.
Fodero-Tavoletti, M.
Huggins, C. E.
Delbridge, L. M.
Zajac, J. D.
Proietto, J.
Journal name Journal of molecular endocrinology
Volume number 31
Issue number 3
Start page 449
End page 459
Total pages 11
Publisher Society for Endocrinology
Place of publication Bristol, England
Publication date 2003
ISSN 0952-5041
Summary The aim of this study was to investigate the metabolic and structural consequences of a decrease in glucose transporter-4 (GLUT4) levels on the heart. The CreLoxP system was utilised to delete GLUT4 in muscle tIssue including heart. The presence of the PGK-neoR cassette in the GLUT4-Lox mice resulted in reduced expression in all tIssues to levels 15-30% of wild-type control mice. In mice expressing Cre recombinase, there was a further reduction of GLUT4 in cardiac tIssue to almost undetectable levels. Cardiac glucose uptake was measured basally and during a uglycaemic/hyperinsulinaemic clamp using 2-deoxy-[1-(14)C]glucose. Insulin-stimulated glucose uptake was normal in hearts expressing 15% of normal GLUT4 levels but markedly reduced in mice with more profound reduction in GLUT4. Cardiac enlargement occurred only when GLUT4 levels were less than 5% of normal values. In heart there is a threshold level of GLUT4 above which insulin-stimulated glucose uptake is maintained. As little as 5% of normal GLUT4 levels expressed in heart is sufficient to prevent the development of cardiac hypertrophy. 2-deoxy-[1-14C]glucose. Insulin-stimulated glucose uptake was normal in hearts expressing 15% of normal GLUT4 levels but markedly reduced in mice with more profound reduction in GLUT4. Cardiac enlargement occurred only when GLUT4 levels were less than 5% of normal values. In heart there is a threshold level of GLUT4 above which insulin-stimulated glucose uptake is maintained. As little as 5% of normal GLUT4 levels expressed in heart is sufficient to prevent the development of cardiac hypertrophy.
Language eng
Field of Research 110306 Endocrinology
110201 Cardiology (incl Cardiovascular Diseases)
Socio Economic Objective 970111
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2003, Society for Endocrinology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30024810

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 25 times in TR Web of Science
Scopus Citation Count Cited 30 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 323 Abstract Views  -  Detailed Statistics
Created: Wed, 03 Mar 2010, 10:41:19 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.