Reverse ascertainment bias in microsatellite allelic diversity in owls (Aves, Strigiformes)

Hogan, Fiona E, Cooke, Raylene. and Norman, Janette A 2009, Reverse ascertainment bias in microsatellite allelic diversity in owls (Aves, Strigiformes), Conservation genetics, vol. 10, no. 3, pp. 635-638, doi: 10.1007/s10592-008-9595-0.

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Title Reverse ascertainment bias in microsatellite allelic diversity in owls (Aves, Strigiformes)
Author(s) Hogan, Fiona E
Cooke, Raylene.ORCID iD for Cooke, Raylene.
Norman, Janette A
Journal name Conservation genetics
Volume number 10
Issue number 3
Start page 635
End page 638
Total pages 4
Publisher Springer Netherlands
Place of publication Dordrecht , Netherlands
Publication date 2009-06
ISSN 1566-0621
Keyword(s) cross-species amplification
Strigidae Tytonidae
reverse ascertainment bias
Summary A rich source of markers may be overlooked by screening for polymorphism in the source species only. We screened 129 microsatellite loci isolated from the powerful owl (Ninox strenua) against two closely related species; Ninox  connivens and Ninox novaeseelandiae. From the screening effort 20 polymorphic markers were isolated, including six loci which were originally discarded as they were monomorphic in the source species. Further cross-species amplification of all 20 loci across species from two families, Strigidae and Tytonidae, revealed unusually high levels of polymorphism within closely related species, and limited success within phylogenetically distant species. Routine screening of multiple  species during the marker development phase can yield a wider range of  polymorphic markers which can subsequently enhance cross-species  amplification attempts.
Language eng
DOI 10.1007/s10592-008-9595-0
Field of Research 060411 Population, Ecological and Evolutionary Genetics
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2008, Springer Science + Business Media B.V.
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