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Exercise increases MEF2- and GEF DNA-binding activity in human skeletal muscle

McGee, Sean L., Sparling, David, Olson, Ann-Louise and Hargreaves, Mark 2006, Exercise increases MEF2- and GEF DNA-binding activity in human skeletal muscle, FASEB journal, vol. 20, no. 2, pp. 348-349.

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Title Exercise increases MEF2- and GEF DNA-binding activity in human skeletal muscle
Author(s) McGee, Sean L.
Sparling, David
Olson, Ann-Louise
Hargreaves, Mark
Journal name FASEB journal
Volume number 20
Issue number 2
Start page 348
End page 349
Publisher Federation of American Societies for Experimental Biology
Place of publication Bethesada, Md.
Publication date 2006-02
ISSN 0892-6638
1530-6860
Summary Diabetes is quickly reaching epidemic proportions, with 216 million people worldwide predicted to be diagnosed with the disease by 2010. While it appears that the expression of the insulin responsive glucose transporter isoform 4 (GLUT4) is not reduced in diabetic populations, overexpression of GLUT4 exclusively in muscle enhances insulin action and improves glucose homeostasis. Consequently, understanding the regulation of GLUT4 expression is considered important in identifying potential therapeutic targets for the treatment and management of insulin resistance and related disorders such as type 2 diabetes. Using transgenic mice, we have identified two conserved regions on the GLUT4 gene promoter that are required for normal skeletal muscle GLUT4 expression. The first region contains a binding site for the myocyte enhancer factor 2 (MEF2) transcription factor, between –464 and –473 bp, and it appears that a MEF2A/D heterodimer binds this sequence. However, this site is not sufficient to support full GLUT4 expression, and another region between –712 and –742 bp, termed Domain 1, is also required. A novel transcription factor, named the GLUT4 enhancer factor (GEF), was found to bind to this region. It appears that MEF2 and GEF physically interact in order to induce GLUT4 expression. A single bout of exercise is sufficient to increase both GLUT4 transcription and mRNA abundance. However, the molecular mechanisms underpinning this response remain largely unexplored, particularly in human skeletal muscle. Therefore, the aim of this study was to determine whether a single, acute bout of exercise increases the DNA-binding activity of both MEF2 and GEF in human skeletal muscle.
Language eng
Field of Research 060114 Systems Biology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2006, FASEB
Persistent URL http://hdl.handle.net/10536/DRO/DU:30025014

Document type: Journal Article
Collection: School of Medicine
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Created: Fri, 12 Mar 2010, 10:40:07 EST by Sean McGee