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A conserved region in the EBL proteins Is implicated in microneme targeting of the malaria parasite Plasmodium falciparum

Treeck, Moritz, Struck, Nicole S., Haase, Silvia, Langer, Christine, Herrmann, Susann, Healer, Julie, Cowman, Alan F. and Gilberger, Tim W. 2006, A conserved region in the EBL proteins Is implicated in microneme targeting of the malaria parasite Plasmodium falciparum, Journal of biological chemistry, vol. 281, no. 42, pp. 31995-32003, doi: 10.1074/jbc.M606717200.

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Title A conserved region in the EBL proteins Is implicated in microneme targeting of the malaria parasite Plasmodium falciparum
Author(s) Treeck, Moritz
Struck, Nicole S.
Haase, Silvia
Langer, Christine
Herrmann, Susann
Healer, Julie
Cowman, Alan F.
Gilberger, Tim W.
Journal name Journal of biological chemistry
Volume number 281
Issue number 42
Start page 31995
End page 32003
Total pages 9
Publisher American Society for Biochemistry and Molecular Biology, Inc.
Place of publication Bethesda, Md.
Publication date 2006
ISSN 0021-9258
1083-351X
Keyword(s) malaria
Summary The proliferation of the malaria parasite Plasmodium falciparum within the human host is dependent upon invasion of erythrocytes. This process is accomplished by the merozoite, a highly specialized form of the parasite. Secretory organelles including micronemes and rhoptries play a pivotal role in the invasion process by storing and releasing parasite proteins. The mechanism of protein sorting to these compartments is unclear. Using a transgenic approach we show that trafficking of the most abundant micronemal proteins (members of the EBL-family: EBA-175, EBA-140/BAEBL, and EBA-181/JSEBL) is independent of their cytoplasmic and transmembrane domains, respectively. To identify the minimal sequence requirements for microneme trafficking, we generated parasites expressing EBAGFP chimeric proteins and analyzed their distribution within the infected erythrocyte. This revealed that: (i) a conserved cysteine-rich region in the ectodomain is necessary for protein trafficking to the micronemes and (ii) correct sorting is dependent on accurate timing of expression.
Language eng
DOI 10.1074/jbc.M606717200
Field of Research 110803 Medical Parasitology
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2006, The American Society for Biochemistry and Molecular Biology, Inc.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30025957

Document type: Journal Article
Collection: School of Medicine
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