The influence of specific luminal factors on the colonic epithelium : high-dose butyrate and physical changes suppress early carcinogenic events in rats

Wong, Cynthia S. M., Sengupta, Shomink, Tjandra, Joe J and Gibson, Peter R. 2005, The influence of specific luminal factors on the colonic epithelium : high-dose butyrate and physical changes suppress early carcinogenic events in rats, Diseases of the colon and rectum, vol. 48, no. 3, pp. 549-559.


Title The influence of specific luminal factors on the colonic epithelium : high-dose butyrate and physical changes suppress early carcinogenic events in rats
Author(s) Wong, Cynthia S. M.
Sengupta, Shomink
Tjandra, Joe J
Gibson, Peter R.
Journal name Diseases of the colon and rectum
Volume number 48
Issue number 3
Start page 549
End page 559
Total pages 11
Publisher Lippincott Williams & Wilkins
Place of publication New York, N.Y.
Publication date 2005-03
ISSN 0012-3706
1530-0358
Summary INTRODUCTION Although luminal delivery of butyrate is one putative mechanism by which biology of the colonic epithelium might be influenced by changes in luminal contents, there is a paucity of supportive cause–effect evidence. This study aimed to directly establish whether distal colonic butyrate delivery is able to alter the response of the distal colonic epithelium to a carcinogen.
METHODS Groups of male Sprague-Dawley rats with chronically intubated colons received infusions of 80 mM butyrate or 0.9 percent saline into distal colon two or five times daily. Three weeks after exposure to azoxymethane (15 mg/kg subcutaneously), the density of aberrant crypts was quantified in distal colon.
RESULTS Infusions of 0.5 ml twice daily, whether containing saline or butyrate, decreased the number of aberrant crypt foci by 45 percent compared with rats receiving no infusions (P = 0.004, analysis of variance). Similar results were obtained when infusions were restricted to the postinitiation phase. When infusions were increased to 1 ml five times daily, saline infusions similarly suppressed aberrant crypt formation (38 percent), but butyrate infusions suppressed it to a greater degree (by 64 percent; P = 0.02 compared with saline infusion, t-test).
CONCLUSIONS High levels of butyrate delivery to the distal colonic lumen alter the epithelial response to a carcinogen in otherwise healthy rats. This finding directly supports the notion that the effects of butyrate on cells in vitro do occur in vivo provided a sufficient dose is delivered. The effect of infusion of liquid per se on the epithelial response highlights the potential impact physical changes alone can have on the colonic epithelium.
Language eng
Field of Research 060602 Animal Physiology - Cell
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2005, The American Society of Colon and Rectal Surgeons
Persistent URL http://hdl.handle.net/10536/DRO/DU:30026158

Document type: Journal Article
Collection: Centre for Material and Fibre Innovation
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