Polyethyleneterephthalate provides superior retention of endothelial cells during shear stress compared to polytetrafluoroethylene and pericardium

Wong, Cynthia S., Sgarioto, Melissa, Owida, Amal A., Yang, William, Rosenfeldt, Franklin L. and Morsi, Yos S. 2006, Polyethyleneterephthalate provides superior retention of endothelial cells during shear stress compared to polytetrafluoroethylene and pericardium, Heart, lung and circulation, vol. 15, no. 6, pp. 371-377.


Title Polyethyleneterephthalate provides superior retention of endothelial cells during shear stress compared to polytetrafluoroethylene and pericardium
Author(s) Wong, Cynthia S.
Sgarioto, Melissa
Owida, Amal A.
Yang, William
Rosenfeldt, Franklin L.
Morsi, Yos S.
Journal name Heart, lung and circulation
Volume number 15
Issue number 6
Start page 371
End page 377
Total pages 7
Publisher Elsevier Australia
Place of publication Chatswood, N.S.W.
Publication date 2006-12
ISSN 1443-9506
Summary Background

Polyethyleneterephthalate (PET) and polytetrafluoroethylene (PTFE) are polymers successfully used as large diameter arterial grafts for peripheral vascular surgery. However, these prosthetic grafts are rarely used for coronary bypass surgery because of their low patency rates. Endothelialisation of the lumenal surface of these materials may improve their patency. This study aimed to compare the endothelialisation of PET, PTFE and pericardium by examining their seeding efficiency over time and the effect of various shear stresses on retention of endothelial cells.

Methods


Ovine endothelial cells at 4 × 105 cells/cm2 were seeded onto PET, PTFE and pericardium, and cultured for 1–168 hours. Cell coverage was determined via en face immunocytochemistry and cell retention was quantified after being subjected to shear stresses ranging from 0.018 to 0.037 N/m2 for 15, 30 and 60 minutes.

Results

Endothelial cells adhered to all of the materials one hour post-seeding. PET exhibited better cell retention rate, ranging from 66.9 ± 5.6% at 0.018 N/m2 for 15 min to 44.7 ± 1.9% at 0.037 N/m2 for 60 minutes, when compared to PTFE and pericardium (p < 0.0001, three-way ANOVA).

Conclusion

PET shows superior retention of endothelial cells during shear stress compare to PTFE and pericardium.

Language eng
Field of Research 090301 Biomaterials
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2006, Australasian Society of Cardiac and Thoracic Surgeons and the Cardiac Society of Australia and New Zealand
Persistent URL http://hdl.handle.net/10536/DRO/DU:30026159

Document type: Journal Article
Collection: Centre for Material and Fibre Innovation
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