Computer-assisted image analysis of liver collagen: relationship to Ishak scoring and hepatic venous pressure gradient

Calvaruso, Vincenza, Burroughs, Andrew Kenneth, Standish, Richard, Manousou, Pinelopi, Grillo, Federica, Leandro, Gioacchino, Maimone, Sergio, Pleguezuelo, Maria, Xirouchakis, Ilias, Guerrini,Gian Piero, Patch, David, Yu, Dominic, O'Beirne, James and Dhillon, Amar Paul 2009, Computer-assisted image analysis of liver collagen: relationship to Ishak scoring and hepatic venous pressure gradient, Hepatology, vol. 49, no. 4, pp. 1236-1244, doi: 10.1002/hep.22745.

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Title Computer-assisted image analysis of liver collagen: relationship to Ishak scoring and hepatic venous pressure gradient
Author(s) Calvaruso, Vincenza
Burroughs, Andrew Kenneth
Standish, Richard
Manousou, Pinelopi
Grillo, Federica
Leandro, Gioacchino
Maimone, Sergio
Pleguezuelo, Maria
Xirouchakis, Ilias
Guerrini,Gian Piero
Patch, David
Yu, Dominic
O'Beirne, James
Dhillon, Amar Paul
Journal name Hepatology
Volume number 49
Issue number 4
Start page 1236
End page 1244
Total pages 9
Publisher John Wiley & Sons, Inc.
Place of publication Hoboken, NJ
Publication date 2009
ISSN 0270-9139
Summary Histopathological scoring of disease stage uses descriptive categories without measuring the amount of fibrosis. Collagen, the major component of fibrous tissue, can be quantified by computer-assisted digital image analysis (DIA) using histological sections. We determined relationships between DIA, Ishak stage, and hepatic venous pressure gradient (HVPG) reflecting severity of fibrosis. One hundred fifteen patients with hepatitis C virus (HCV) who had undergone transplantation had 250 consecutive transjugular liver biopsies combined with HVPG (median length, 22 mm; median total portal tracts, 12), evaluated using the Ishak system and stained with Sirus red for DIA. Liver collagen was expressed as collagen proportionate area (CPA). Median CPA was 6% (0.2-45), correlating with Ishak stage (stage 6 range, 13%-45%), and with HVPG (r = 0.62; P < 0.001). Median CPA was 4.1% when HVPG was less than 6 mm Hg and 13.8% when HVPG was 6 mm Hg or more (P < 0.0001) and 6% when HVPG was less than 10 mm Hg and 17.3% when HVPG was 10 mm Hg or higher (P < 0.0001). Only CPA, not Ishak stage/grade, was independently associated by logistic regression, with HVPG of 6 mm Hg or more [odds ratio, 1.206; 95% confidence interval (CI), 1.094-1.331; P < 0.001], or HVPG of 10 mm Hg or more (odds ratio, 1.105; 95% CI, 1.026-1.191; P = 0.009). CPA increased by 50% (3.6%) compared with 20% in HVPG (1 mm Hg) in 38 patients with repeated biopsies. Conclusion: CPA assessed by DIA correlated with Ishak stage scores and HVPG measured contemporaneously. CPA was a better histological correlate with HVPG than Ishak stage, had a greater numerical change when HVPG was low, and resulted in further quantitation of fibrosis in cirrhosis.
Language eng
DOI 10.1002/hep.22745
Field of Research 110202 Haematology
Socio Economic Objective 920101 Blood Disorders
HERDC Research category CN.1 Other journal article
Copyright notice ©2008, by the American Association for the Study of Liver Diseases
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Document type: Journal Article
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