The role of osmiophilic bodies and Pfg377 expression in female gametocyte emergence and mosquito infectivity in the human malaria parasite plasmodium falciparum

de Koning-Ward, Tania F., Olivieri, Anna, Bertuccini, Lucia, Hood, Andrew, Silvestrini, Francesco, Charvalias, Konstantinos, Diaz, Pedro Berzosa, Camarda, Grazia, McElwain, Terry F., Papenfuss, Tony, Healer, Julie, Baldassarri, Lucilla, Crabb, Brendan S., Alano, Pietro and Ranford-Cartwright, Lisa C. 2008, The role of osmiophilic bodies and Pfg377 expression in female gametocyte emergence and mosquito infectivity in the human malaria parasite plasmodium falciparum, Molecular microbiology, vol. 67, no. 2, pp. 278-290.

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Title The role of osmiophilic bodies and Pfg377 expression in female gametocyte emergence and mosquito infectivity in the human malaria parasite plasmodium falciparum
Author(s) de Koning-Ward, Tania F.
Olivieri, Anna
Bertuccini, Lucia
Hood, Andrew
Silvestrini, Francesco
Charvalias, Konstantinos
Diaz, Pedro Berzosa
Camarda, Grazia
McElwain, Terry F.
Papenfuss, Tony
Healer, Julie
Baldassarri, Lucilla
Crabb, Brendan S.
Alano, Pietro
Ranford-Cartwright, Lisa C.
Journal name Molecular microbiology
Volume number 67
Issue number 2
Start page 278
End page 290
Total pages 13
Publisher Wiley-Blackwell
Place of publication Oxford, England
Publication date 2008-01
ISSN 0950-382X
1365-2958
Keyword(s) malaria
Summary Osmiophilic bodies are membrane-bound vesicles, found predominantly in Plasmodium female gametocytes, that become progressively more abundant as the gametocyte reaches full maturity. These vesicles lie beneath the subpellicular membrane of the gametocyte, and the release of their contents into the parasitophorous vacuole has been postulated to aid in the escape of gametocytes from the erythrocyte after ingestion by the mosquito. Currently, the only protein known to be associated with osmiophilic bodies in Plasmodium falciparum is Pfg377, a gametocyte-specific protein expressed at the onset of osmiophilic body development. Here we show by targeted gene disruption that Pfg377 plays a fundamental role in the formation of these organelles, and that female gametocytes lacking the full complement of osmiophilic bodies are significantly less efficient both in vitro and in vivo in their emergence from the erythrocytes upon induction of gametogenesis, a process whose timing is critical for fertilization with the short-lived male gamete. This reduced efficiency of emergence explains the significant defect in oocyst formation in mosquitoes fed blood meals containing Pfg377-negative gametocytes, resulting in an almost complete blockade of infection.
Language eng
Field of Research 110803 Medical Parasitology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2007, Wiley-Blackwell Publishing
Persistent URL http://hdl.handle.net/10536/DRO/DU:30028156

Document type: Journal Article
Collection: School of Medicine
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