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Using omeprazole to link the components of the post-prandial alkaline tide in the spiny dogfish, Squalus acanthias

Wood, Chris M., Schultz, Aaron G., Munger, Stephen R. and Walsh, Patrick J. 2009, Using omeprazole to link the components of the post-prandial alkaline tide in the spiny dogfish, Squalus acanthias, Journal of experimental biology, vol. 212, no. 5, pp. 684-692, doi: 10.1242/jeb.026450.

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Title Using omeprazole to link the components of the post-prandial alkaline tide in the spiny dogfish, Squalus acanthias
Formatted title Using omeprazole to link the components of the post-prandial alkaline tide in the spiny dogfish, Squalus acanthias
Author(s) Wood, Chris M.
Schultz, Aaron G.ORCID iD for Schultz, Aaron G. orcid.org/0000-0002-7333-7646
Munger, Stephen R.
Walsh, Patrick J.
Journal name Journal of experimental biology
Volume number 212
Issue number 5
Start page 684
End page 692
Publisher Company of Biologists Ltd.
Place of publication Cambridge, England
Publication date 2009-03
ISSN 0022-0949
1477-9145
Keyword(s) feeding
shark
gastric acid secretion
branchial base excretion
chyme composition
metabolic alkalosis
Summary After a meal, dogfish exhibit a metabolic alkalosis in the bloodstream and a marked excretion of basic equivalents across the gills to the external seawater. We used the H+, K+-ATPase pump inhibitor omeprazole to determine whether these post-prandial alkaline tide events were linked to secretion of H+ (accompanied by Cl) in the stomach. Sharks were fitted with indwelling stomach tubes for pretreatment with omeprazole (five doses of 5mg omeprazole per kilogram over 48 h) or comparable volumes of vehicle (saline containing 2% DMSO) and for sampling of gastric chyme. Fish were then fed an involuntary meal by means of the stomach tube consisting of minced flatfish muscle (2% of body mass) suspended in saline (4% of body mass total volume). Omeprazole pretreatment delayed the post-prandial acidification of the gastric chyme, slowed the rise in Cl concentration of the chyme and altered the patterns of other ions, indicating inhibition of H+ and accompanying Clsecretion. Omeprazole also greatly attenuated the rise in arterial pH and bicarbonate concentrations and reduced the net excretion of basic equivalents to the water by 56% over 48h. Arterial blood CO2 pressure and plasma ions were not substantially altered. These results indicate that elevated gastric H+ secretion (as HCl) in the digestive process is the major cause of the systemic metabolic alkalosis and the accompanying rise in base excretion across the gills that constitute the alkaline tide in the dogfish.
Language eng
DOI 10.1242/jeb.026450
Field of Research 060604 Comparative Physiology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30028366

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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Created: Fri, 16 Apr 2010, 16:51:18 EST by Teresa Treffry

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