Diversity and clonotypic composition of influenza-specific CD8+ TCR repertoires remain unaltered in the absence of Aire

Kedzierska, Katherine, Valkenburg, Sophie A., Guillonneau, Carole, Hubert, Francois-Xavier, Cukalac, Tania, Curtis, Joan M., Stambas, John, Scott, Hamish S., Kedzierski, Lukasz, Venturi, Vanessa and Davenport, Miles P. 2010, Diversity and clonotypic composition of influenza-specific CD8+ TCR repertoires remain unaltered in the absence of Aire, European journal of immunology, vol. 40, no. 3, pp. 849-858.

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Title Diversity and clonotypic composition of influenza-specific CD8+ TCR repertoires remain unaltered in the absence of Aire
Author(s) Kedzierska, Katherine
Valkenburg, Sophie A.
Guillonneau, Carole
Hubert, Francois-Xavier
Cukalac, Tania
Curtis, Joan M.
Stambas, John
Scott, Hamish S.
Kedzierski, Lukasz
Venturi, Vanessa
Davenport, Miles P.
Journal name European journal of immunology
Volume number 40
Issue number 3
Start page 849
End page 858
Total pages 10
Publisher Wiley - V C H Verlag GmbH & Co. KGaA
Place of publication Weinheim, Germany
Publication date 2010
ISSN 0014-2980
1521-4141
Keyword(s) CD8+ T cells
influenza
T-cell receptor repertoire
viral infection
Summary TCR repertoire diversity is important for the protective efficacy of CD8+ T cells, limiting viral escape and cross-reactivity between unrelated epitopes. The exact mechanism for selection of restricted versus diverse TCR repertoires is far from clear, although one thought is that the epitopes resembling self-peptides might select a limited array of TCR due to the deletion of autoreactive TCR. The molecule Aire promotes the expression of tissue-specific Ag on thymic medullary epithelial cells and the deletion of autoreactive cells, and in the absence of Aire autoreactive cells persist. However, the contribution of Aire-dependent peptides to the selection of the Ag-specific TCR repertoire remains unknown. In this study, we dissect restricted (DbNP366%+CD8+) and diverse (DbPA224%+CD8+, KdNP147%+CD8+) TCR repertoires responding to three influenza-derived peptides in Aire-deficient mice on both B6 and BALB/c backgrounds. Our study shows that the number, qualitative characteristics and TCR repertoires of all influenza-specific, DbNP366%+CD8+, DbPA224%+CD8+ and KdNP147%+CD8+ T cells are not significantly altered in the absence of Aire. This provides the first demonstration that the selection of an Ag-specific T-cell repertoire is not significantly perturbed in the absence of Aire.
Language eng
Field of Research 110309 Infectious Diseases
Socio Economic Objective 920109 Infectious Diseases
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2009, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Persistent URL http://hdl.handle.net/10536/DRO/DU:30028854

Document type: Journal Article
Collection: School of Medicine
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