Diversity and clonotypic composition of influenza-specific CD8+ TCR repertoires remain unaltered in the absence of Aire

Diversity and clonotypic composition of influenza-specific CD8+ TCR repertoires remain unaltered in the absence of Aire

Kedzierska, Katherine, Valkenburg, Sophie A., Guillonneau, Carole, Hubert, Francois-Xavier, Cukalac, Tania, Curtis, Joan M., Stambas, John, Scott, Hamish S., Kedzierski, Lukasz, Venturi, Vanessa and Davenport, Miles P. 2010, Diversity and clonotypic composition of influenza-specific CD8+ TCR repertoires remain unaltered in the absence of Aire, European journal of immunology, vol. 40, no. 3, pp. 849-858.

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Title	Diversity and clonotypic composition of influenza-specific CD8+ TCR repertoires remain unaltered in the absence of Aire
Author(s)	Kedzierska, Katherine Valkenburg, Sophie A. Guillonneau, Carole Hubert, Francois-Xavier Cukalac, Tania Curtis, Joan M. Stambas, John Scott, Hamish S. Kedzierski, Lukasz Venturi, Vanessa Davenport, Miles P.
Journal name	European journal of immunology
Volume number	40
Issue number	3
Start page	849
End page	858
Publisher	Wiley - V C H Verlag GmbH & Co. KGaA
Place of publication	Weinheim, Germany
Publication date	2010
ISSN	0014-2980 1521-4141
Keyword(s)	CD8+ T cells influenza T-cell receptor repertoire viral infection
Summary	TCR repertoire diversity is important for the protective efficacy of CD8⁺ T cells, limiting viral escape and cross-reactivity between unrelated epitopes. The exact mechanism for selection of restricted versus diverse TCR repertoires is far from clear, although one thought is that the epitopes resembling self-peptides might select a limited array of TCR due to the deletion of autoreactive TCR. The molecule Aire promotes the expression of tissue-specific Ag on thymic medullary epithelial cells and the deletion of autoreactive cells, and in the absence of Aire autoreactive cells persist. However, the contribution of Aire-dependent peptides to the selection of the Ag-specific TCR repertoire remains unknown. In this study, we dissect restricted (D^bNP₃₆₆%⁺CD8⁺) and diverse (D^bPA₂₂₄%⁺CD8⁺, K^dNP₁₄₇%⁺CD8⁺) TCR repertoires responding to three influenza-derived peptides in Aire-deficient mice on both B6 and BALB/c backgrounds. Our study shows that the number, qualitative characteristics and TCR repertoires of all influenza-specific, D^bNP₃₆₆%⁺CD8⁺, D^bPA₂₂₄%⁺CD8⁺ and K^dNP₁₄₇%⁺CD8⁺ T cells are not significantly altered in the absence of Aire. This provides the first demonstration that the selection of an Ag-specific T-cell repertoire is not significantly perturbed in the absence of Aire.
Language	eng
Field of Research	110309 Infectious Diseases
Socio Economic Objective	920109 Infectious Diseases
HERDC Research category	C1.1 Refereed article in a scholarly journal
Copyright notice	©2009, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Persistent URL	http://hdl.handle.net/10536/DRO/DU:30028854

Document type:	Journal Article
Collection:	School of Medicine

Citation counts:	Cited 2 times in TR Web of Science Cited 2 times in Scopus
Access Statistics:	292 Abstract Views, 0 File Downloads - Detailed Statistics
Created:	Wed, 26 May 2010, 16:06:24 EST

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Diversity and clonotypic composition of influenza-specific CD8+ TCR repertoires remain unaltered in the absence of Aire

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