Diversity and clonotypic composition of influenza-specific CD8+ TCR repertoires remain unaltered in the absence of Aire

Kedzierska, Katherine, Valkenburg, Sophie A., Guillonneau, Carole, Hubert, Francois-Xavier, Cukalac, Tania, Curtis, Joan M., Stambas, John, Scott, Hamish S., Kedzierski, Lukasz, Venturi, Vanessa and Davenport, Miles P. 2010, Diversity and clonotypic composition of influenza-specific CD8+ TCR repertoires remain unaltered in the absence of Aire, European journal of immunology, vol. 40, no. 3, pp. 849-858, doi: 10.1002/eji.200939918.

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Title Diversity and clonotypic composition of influenza-specific CD8+ TCR repertoires remain unaltered in the absence of Aire
Author(s) Kedzierska, Katherine
Valkenburg, Sophie A.
Guillonneau, Carole
Hubert, Francois-Xavier
Cukalac, Tania
Curtis, Joan M.
Stambas, JohnORCID iD for Stambas, John orcid.org/0000-0002-5690-2551
Scott, Hamish S.
Kedzierski, Lukasz
Venturi, Vanessa
Davenport, Miles P.
Journal name European journal of immunology
Volume number 40
Issue number 3
Start page 849
End page 858
Total pages 10
Publisher Wiley - V C H Verlag GmbH & Co. KGaA
Place of publication Weinheim, Germany
Publication date 2010
ISSN 0014-2980
Keyword(s) CD8+ T cells
T-cell receptor repertoire
viral infection
Summary TCR repertoire diversity is important for the protective efficacy of CD8+ T cells, limiting viral escape and cross-reactivity between unrelated epitopes. The exact mechanism for selection of restricted versus diverse TCR repertoires is far from clear, although one thought is that the epitopes resembling self-peptides might select a limited array of TCR due to the deletion of autoreactive TCR. The molecule Aire promotes the expression of tissue-specific Ag on thymic medullary epithelial cells and the deletion of autoreactive cells, and in the absence of Aire autoreactive cells persist. However, the contribution of Aire-dependent peptides to the selection of the Ag-specific TCR repertoire remains unknown. In this study, we dissect restricted (DbNP366%+CD8+) and diverse (DbPA224%+CD8+, KdNP147%+CD8+) TCR repertoires responding to three influenza-derived peptides in Aire-deficient mice on both B6 and BALB/c backgrounds. Our study shows that the number, qualitative characteristics and TCR repertoires of all influenza-specific, DbNP366%+CD8+, DbPA224%+CD8+ and KdNP147%+CD8+ T cells are not significantly altered in the absence of Aire. This provides the first demonstration that the selection of an Ag-specific T-cell repertoire is not significantly perturbed in the absence of Aire.
Language eng
DOI 10.1002/eji.200939918
Field of Research 110309 Infectious Diseases
Socio Economic Objective 920109 Infectious Diseases
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2009, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Persistent URL http://hdl.handle.net/10536/DRO/DU:30028854

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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