Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes

Andreansky, S., Stambas, J., Gutierrez, A., Diaz, G., Doherty, P.C., Turner, S.J. and Webby, R.J. 2004, Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes, International Congress Series, vol. 1263, pp. 141-144.

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Title Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes
Author(s) Andreansky, S.
Stambas, J.
Gutierrez, A.
Diaz, G.
Doherty, P.C.
Turner, S.J.
Webby, R.J.
Journal name International Congress Series
Volume number 1263
Start page 141
End page 144
Publisher Elsevier BV
Place of publication The Netherlands
Publication date 2004-06
ISSN 0531-5131
Keyword(s) Influenza
Cytotoxic
CD8
Epitope
Summary The induction of a cytotoxic T lymphocyte (CTL) response following influenza infection can lead to the formation of immunity capable of recognizing viruses of a different antigenicity. Our ability to exploit such broadly reactive responses in vaccination strategies is hampered by a lack of understanding on the regulation of CTL responses. In this report, we describe the utilization of reverse genetics to produce a range of recombinant viruses lacking immunodominant murine CTL epitopes. Recombinant viruses lacking the epitopes had indistinguishable growth properties in vitro and in vivo compared with the wild-type virus. Analysis of a primary immune response to these viruses showed that mutation of the anchor-binding residue leads to a loss of a response to that epitope, but no compensating increase in responses to other immunodominant epitopes. The utilization of reverse genetics and the murine model of influenza infection hold great promise for elucidating the factors regulating the CTL response.
Language eng
Field of Research 110804 Medical Virology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ┬ęCopyright 2004 Elsevier B.V. All rights reserved.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30028907

Document type: Journal Article
Collection: School of Medicine
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