Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes

Andreansky, S., Stambas, J., Gutierrez, A., Diaz, G., Doherty, P.C., Turner, S.J. and Webby, R.J. 2004, Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes, International Congress Series, vol. 1263, pp. 141-144, doi: 10.1016/j.ics.2004.01.046.

Attached Files
Name Description MIMEType Size Downloads

Title Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes
Author(s) Andreansky, S.
Stambas, J.ORCID iD for Stambas, J.
Gutierrez, A.
Diaz, G.
Doherty, P.C.
Turner, S.J.
Webby, R.J.
Journal name International Congress Series
Volume number 1263
Start page 141
End page 144
Publisher Elsevier BV
Place of publication The Netherlands
Publication date 2004-06
ISSN 0531-5131
Keyword(s) Influenza
Summary The induction of a cytotoxic T lymphocyte (CTL) response following influenza infection can lead to the formation of immunity capable of recognizing viruses of a different antigenicity. Our ability to exploit such broadly reactive responses in vaccination strategies is hampered by a lack of understanding on the regulation of CTL responses. In this report, we describe the utilization of reverse genetics to produce a range of recombinant viruses lacking immunodominant murine CTL epitopes. Recombinant viruses lacking the epitopes had indistinguishable growth properties in vitro and in vivo compared with the wild-type virus. Analysis of a primary immune response to these viruses showed that mutation of the anchor-binding residue leads to a loss of a response to that epitope, but no compensating increase in responses to other immunodominant epitopes. The utilization of reverse genetics and the murine model of influenza infection hold great promise for elucidating the factors regulating the CTL response.
Language eng
DOI 10.1016/j.ics.2004.01.046
Field of Research 110804 Medical Virology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ┬ęCopyright 2004 Elsevier B.V. All rights reserved.
Persistent URL

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Connect to link resolver
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in TR Web of Science
Scopus Citation Count Cited 1 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 675 Abstract Views, 0 File Downloads  -  Detailed Statistics
Created: Wed, 26 May 2010, 16:07:15 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact