An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8+ T cells

Stambas, John, Doherty, Peter C. and Turner, Stephen J. 2007, An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8+ T cells, The Journal of Immunology, vol. 178, no. 3, pp. 1285-1292.

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Title An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8+ T cells
Author(s) Stambas, John
Doherty, Peter C.
Turner, Stephen J.
Journal name The Journal of Immunology
Volume number 178
Issue number 3
Start page 1285
End page 1292
Publisher American Association of Immunologists
Place of publication Baltimore, Md.
Publication date 2007-02-01
ISSN 0022-1767
1550-6606
Summary Influenza A virus infection of C57BL/6 (B6) mice is characterized by prominent CD8 T cell responses to H2Db complexed with peptides from the viral nucleoprotein (NP366, ASNENMETM) and acid polymerase (PA224, SSLENFRAYV). An in vivo cytotoxicity assay that depends on the adoptive transfer of peptide-pulsed, syngeneic targets was used in this study to quantitate the cytotoxic potential of DbNP366- and DbPA224-specific acute and memory CD8 T cells following primary or secondary virus challenge. Both T cell populations displayed equivalent levels of in vivo effector function when comparable numbers were transferred into naive B6 hosts. Cytotoxic activity following primary infection clearly correlated with the frequency of tetramer-stained CD8 T cells. This relationship looked, however, to be less direct following secondary exposure, partly because the numbers of CD8DbNP366 T cells were greatly in excess. However, calculating the in vivo E:T ratios indicated that in vivo lysis, like many other biological functions, is threshold dependent. Furthermore, the capacity to eliminate peptide-pulsed targets was independent of the differentiation state (i.e., primary or secondary effectors) and was comparable for the two T cell specificities that were analyzed. These experiments provide insights that may be of value for adoptive immunotherapy, where careful consideration of both the activation state and the number of effector cells is required.
Language eng
Field of Research 110804 Medical Virology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ┬ęCopyright 2007 by The American Association of Immunologists, Inc. All rights reserved.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30028910

Document type: Journal Article
Collection: School of Medicine
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