Enhanced proliferation of human skeletal muscle precursor cells derived from elderly donors cultured in estimated physiological (5%) oxygen

Martin, Sheree D., Collier, Fiona M., Kirkland, Mark A., Walder, Ken and Stupka, Nicole 2009, Enhanced proliferation of human skeletal muscle precursor cells derived from elderly donors cultured in estimated physiological (5%) oxygen, Cytotechnology: international journal of cell culture and biotechnology, vol. 61, pp. 93-107, doi: 10.1007/s10616-009-9247-3.

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Title Enhanced proliferation of human skeletal muscle precursor cells derived from elderly donors cultured in estimated physiological (5%) oxygen
Author(s) Martin, Sheree D.
Collier, Fiona M.ORCID iD for Collier, Fiona M. orcid.org/0000-0002-5438-480X
Kirkland, Mark A.
Walder, KenORCID iD for Walder, Ken orcid.org/0000-0002-6758-4763
Stupka, NicoleORCID iD for Stupka, Nicole orcid.org/0000-0002-1000-1707
Journal name Cytotechnology: international journal of cell culture and biotechnology
Volume number 61
Start page 93
End page 107
Total pages 15
Publisher Springer Netherlands
Place of publication Dordrecht, Netherlands
Publication date 2009
ISSN 0920-9069
Keyword(s) Human primary myoblasts
Cell cycle
Species differences
Summary Human skeletal muscle precursor cells (myoblasts) have significant therapeutic potential and are a valuable research tool to study muscle cell biology. Oxygen is a critical factor in the successful culture of myoblasts with low (1–6%) oxygen culture conditions enhancing the proliferation, differentiation, and/or viability of mouse, rat, and bovine myoblasts. The specific effects of low oxygen depend on the myoblast source and oxygen concentration; however, variable oxygen conditions have not been tested in the culture of human myoblasts. In this study, muscle precursor cells were isolated from vastus lateralis muscle biopsies and myoblast cultures were established in 5% oxygen, before being divided into physiological (5%) or standard (20%) oxygen conditions for experimental analysis. Five percent oxygen increased proliferating myoblast numbers, and since low oxygen had no significant effect on myoblast viability, this increase in cell number was attributed to enhanced proliferation. The proportion of cells in the S (DNA synthesis) phase of the cell cycle was increased by 50%, and p21Cip1 gene and protein expression was decreased in 5 versus 20% oxygen. Unlike in rodent and bovine myoblasts, the increase in myoD, myogenin, creatine kinase, and myosin heavy chain IIa gene expression during differentiation was similar in 5 and 20% oxygen; as was myotube hypertrophy. These data indicate for the first time that low oxygen culture conditions stimulate proliferation, whilst maintaining (but not enhancing) the viability and the differentiation potential of human primary myoblasts and should be considered as optimum conditions for exvivo expansion of these cells.

Language eng
DOI 10.1007/s10616-009-9247-3
Field of Research 110106 Medical Biochemistry: Proteins and Peptides (incl Medical Proteomics)
Socio Economic Objective 920104 Diabetes
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2009
Copyright notice ©2010, Springer Science+Business Media B.V.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30029022

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