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Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone

Panasophonkul, Sasiporn, Apisawetakan, Somjai, Cummins, Scott F., York, Patrick S., Degnan, Bernard M., Hanna, Peter J., Saitongdee, Porncharn, Sobhon, Prasert and Sretarugsa, Prapee 2009, Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone, Histochemistry and Cell Biology, vol. 131, pp. 629-642, doi: 10.1007/s00418-009-0555-7.

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Title Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone
Author(s) Panasophonkul, Sasiporn
Apisawetakan, Somjai
Cummins, Scott F.
York, Patrick S.
Degnan, Bernard M.
Hanna, Peter J.
Saitongdee, Porncharn
Sobhon, Prasert
Sretarugsa, Prapee
Journal name Histochemistry and Cell Biology
Volume number 131
Start page 629
End page 642
Publisher Springer
Place of publication Heidelberg, Germany
Publication date 2009-05
ISSN 0948-6143
1432-119X
Keyword(s) Serotonin receptor
Abalone
Ganglia
Gonads
Summary In molluscs, the neurotransmitter serotonin (5-HT) has been linked to a variety of biological roles including gamete maturation and spawning. The possible involvement of 5-HT in abalone gamete release was demonstrated by a dose-dependent increase in Haliotis rubra gonad contractile bioactivity following 5-HT stimulation. Physiological functions associated with 5-HT, are mediated through binding to 5-HT receptors. A cDNA encoding a putative 5-HT receptor consisting of 359 amino acids was isolated from the tropical abalone H. asinina, termed 5-HT1 ha. The 5-HT1 ha shares G-protein-coupled receptor motifs with metazoan 5-HT receptors, including predicted transmembrane domains, active sites for protein kinase action, and N-linked glycosylation sites. However, the third intracellular loop of 5-HT1 ha is relatively short, and only six transmembrane domains are predicted, implying a truncated receptor. Phylogenetic analysis with known 5-HT receptor genes suggests that 5-HT1 ha belongs to the type 1 5-HT receptor family. Expression analysis by RT-PCR showed that 5-HT1 ha  mRNA was present in all tissues examined, including the neural ganglia and gonad tissues. Immunocytochemistry revealed the presence of 5-HT1 ha specifically within the soma of neuronal cells located in the outer cortex of both cerebral and pleuropedal ganglia. In ovarian and testicular tissues, 5-HT1 ha immunoreactivity was observed in epithelial cells of the outer capsule and connective tissue of the trabeculae to which the gamete follicles adhere. Whether this receptor transcript is translated to a functional protein needs to be verified, but if so, it could play a role in reproduction.

Language eng
DOI 10.1007/s00418-009-0555-7
Field of Research 060105 Cell Neurochemistry
Socio Economic Objective 830103 Aquaculture Molluscs (excl. Oysters)
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2009
Copyright notice ©2009, Springer-Verlag
Persistent URL http://hdl.handle.net/10536/DRO/DU:30029091

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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Created: Fri, 04 Jun 2010, 16:55:48 EST by Teresa Treffry

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