A microfluidics device to monitor platelet aggregation dynamics in response to strain rate micro-gradients in flowing blood

Tovar-Lopez, Francisco Javier, Rosengarten, Gary, Westein, Erik, Khoshmanesh, Khashayar, Jackson, Shaun P., Mitchell, Arnan and Nesbitt, Warwick S. 2010, A microfluidics device to monitor platelet aggregation dynamics in response to strain rate micro-gradients in flowing blood, Lab on a chip, vol. 10, no. 3, pp. 291-302.

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Title A microfluidics device to monitor platelet aggregation dynamics in response to strain rate micro-gradients in flowing blood
Author(s) Tovar-Lopez, Francisco Javier
Rosengarten, Gary
Westein, Erik
Khoshmanesh, Khashayar
Jackson, Shaun P.
Mitchell, Arnan
Nesbitt, Warwick S.
Journal name Lab on a chip
Volume number 10
Issue number 3
Start page 291
End page 302
Publisher Royal Society of Chemistry
Place of publication London, England
Publication date 2010
ISSN 1473-0197
1473-0189
Summary This paper reports the development of a platform technology for measuring platelet function and aggregation based on localized strain rate micro-gradients. Recent experimental findings within our laboratories have identified a key role for strain rate micro-gradients in focally triggering initial recruitment and subsequent aggregation of discoid platelets at sites of blood vessel injury. We present the design justification, hydrodynamic characterization and experimental validation of a microfluidic device incorporating contraction–expansion geometries that generate strain rate conditions mimicking the effects of pathological changes in blood vessel geometry. Blood perfusion through this device supports our published findings of both in vivo and in vitro platelet aggregation and confirms a critical requirement for the coupling of blood flow acceleration to downstream deceleration for the initiation and stabilization of platelet aggregation, in the absence of soluble platelet agonists. The microfluidics platform presented will facilitate the detailed analysis of the effects of hemodynamic parameters on the rate and extent of platelet aggregation and will be a useful tool to elucidate the hemodynamic and platelet mechano-transduction mechanisms, underlying this shear-dependent process.
Language eng
Field of Research 091306 Microelectromechanical Systems (MEMS)
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2010
Copyright notice ©2010, The Royal Society of Chemistry
Persistent URL http://hdl.handle.net/10536/DRO/DU:30029168

Document type: Journal Article
Collection: Centre for Intelligent Systems Research
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