Zebrafish granulocyte colony-stimulatingZebrafish granulocyte colony-stimulating factor receptor signaling promotes myelopoiesis and myeloid cell migration

Liongue, Clifford, Hall, Chris J., O'Connell, Bree A., Crosier, Phil and Ward, Alister C. 2009, Zebrafish granulocyte colony-stimulatingZebrafish granulocyte colony-stimulating factor receptor signaling promotes myelopoiesis and myeloid cell migration, Blood, vol. 113, no. 11, pp. 2535-2546.

Attached Files
Name Description MIMEType Size Downloads

Title Zebrafish granulocyte colony-stimulatingZebrafish granulocyte colony-stimulating factor receptor signaling promotes myelopoiesis and myeloid cell migration
Author(s) Liongue, Clifford
Hall, Chris J.
O'Connell, Bree A.
Crosier, Phil
Ward, Alister C.
Journal name Blood
Volume number 113
Issue number 11
Start page 2535
End page 2546
Total pages 12
Publisher American Society of Hematology
Place of publication Washington, D.C.
Publication date 2009-03-12
ISSN 0006-4971
1528-0020
Summary Granulocyte colony-stimulating factor receptor (GCSFR) signaling participates in the production of neutrophilic granulocytes during normal hematopoietic development, with a particularly important role during emergency hematopoiesis. This study describes the characterization of the zebrafish gcsf and gcsfr genes, which showed broad conservation and similar regulation to their mammalian counterparts. Morpholino-mediated knockdown of gcsfr and overexpression of gcsf revealed the presence of an anterior population of myeloid cells during primitive hematopoiesis that was dependent on GCSF/GCSFR for development and migration. This contrasted with a posterior domain that was largely independent of this pathway. Definitive myelopoiesis was also partially dependent on a functional GCSF/GCSFR pathway. Injection of bacterial lipopolysaccharide elicited significant induction of gcsf expression and emergency production of myeloid cells, which was abrogated by gcsfr knockdown. Collectively, these data demonstrate GCSF/GCSFR to be a conserved signaling system for facilitating the production of multiple myeloid cell lineages in both homeostatic and emergency conditions, as well as for early myeloid cell migration, establishing a useful experimental platform for further dissection of this pathway.
Language eng
Field of Research 110707 Innate Immunity
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2009, The American Society of Hematology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30029770

Document type: Journal Article
Collection: School of Medicine
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 35 times in TR Web of Science
Scopus Citation Count Cited 36 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 301 Abstract Views, 1 File Downloads  -  Detailed Statistics
Created: Mon, 23 Aug 2010, 12:49:05 EST by Jane Moschetti

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.