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Molecular genetics and comparative genomics reveal RNAi is not functional in malaria parasites Baum, Jake, Papenfuss, Anthony T., Mair, Gunnar R., Janse, Chris J., Vlachou, Dina, Waters, Andrew P., Cowman, Alan F., Crabb, Brendan S. and de Koning-Ward, Tania F. 2009, Molecular genetics and comparative genomics reveal RNAi is not functional in malaria parasites, Nucleic acids research advance access, vol. 37, no. 11, pp. 3788-3798. Attached Files Name Description MIMEType Size Downloads dekoningward-moleculargenetics-2009.pdf Published version application/pdf 1004.64KB 7 Title Molecular genetics and comparative genomics reveal RNAi is not functional in malaria parasites Author(s) Baum, Jake Papenfuss, Anthony T. Mair, Gunnar R. Janse, Chris J. Vlachou, Dina Waters, Andrew P. Cowman, Alan F. Crabb, Brendan S. de Koning-Ward, Tania F. Journal name Nucleic acids research advance access Volume number 37 Issue number 11 Start page 3788 End page 3798 Total pages 12 Publisher Oxford University Press Place of publication Oxford, England Publication date 2009-06 ISSN 1362-4954 1362-4962 0261-3166 0952-3472 Keyword(s) malaria Summary Techniques for targeted genetic disruption in Plasmodium, the causative agent of malaria, are currently intractable for those genes that are essential for blood stage development. The ability to use RNA interference (RNAi) to silence gene expression would provide a powerful means to gain valuable insight into the pathogenic blood stages but its functionality in Plasmodium remains controversial. Here we have used various RNA-based gene silencing approaches to test the utility of RNAi in malaria parasites and have undertaken an extensive comparative genomics search using profile hidden Markov models to clarify whether RNAi machinery exists in malaria. These investigative approaches revealed that Plasmodium lacks the enzymology required for RNAi-based ablation of gene expression and indeed no experimental evidence for RNAi was observed. In its absence, the most likely explanations for previously reported RNAi-mediated knockdown are either the general toxicity of introduced RNA (with global down-regulation of gene expression) or a specific antisense effect mechanistically distinct from RNAi, which will need systematic analysis if it is to be of use as a molecular genetic tool for malaria parasites. Notes Published online 20 April 2009 Language eng Field of Research 060407 Genome Structure and Regulation Socio Economic Objective 920109 Infectious Diseases HERDC Research category C1.1 Refereed article in a scholarly journal Copyright notice ©2009, The Authors Persistent URL http://hdl.handle.net/10536/DRO/DU:30029776 Document type: Journal Article Collections: School of Medicine Open Access Collection Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Mon, 23 Aug 2010, 14:11:18 EST Tue, 24 Aug 2010, 08:20:34 EST Wed, 25 Aug 2010, 08:33:32 EST Wed, 25 Aug 2010, 12:39:50 EST Fri, 27 Aug 2010, 08:37:36 EST Mon, 06 Sep 2010, 12:20:01 EST Mon, 06 Sep 2010, 12:24:16 EST Mon, 06 Sep 2010, 12:56:03 EST Mon, 06 Sep 2010, 13:02:37 EST Mon, 06 Sep 2010, 13:04:10 EST Fri, 24 Feb 2012, 15:01:19 EST Wed, 11 Sep 2013, 09:24:01 EST Thu, 12 Sep 2013, 09:06:19 EST Mon, 16 Sep 2013, 12:54:25 EST Mon, 16 Sep 2013, 12:56:01 EST Filtered Full Citation counts:   Cited 54 times in TR Web of Science Cited 57 times in Scopus Search Google Scholar Access Statistics: 332 Abstract Views, 8 File Downloads  -  Detailed Statistics Created: Mon, 23 Aug 2010, 14:11:13 EST by Jane Moschetti

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.