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Suppressive actions of eicosapentaenoic acid on lipid droplet formation in 3T3-L1 adipocytes

Manickam, Elizabeth, Sinclair, Andrew J. and Cameron-Smith, David 2010, Suppressive actions of eicosapentaenoic acid on lipid droplet formation in 3T3-L1 adipocytes, Lipids in health and disease, vol. 9, pp. 1-8.

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Title Suppressive actions of eicosapentaenoic acid on lipid droplet formation in 3T3-L1 adipocytes
Author(s) Manickam, Elizabeth
Sinclair, Andrew J.
Cameron-Smith, David
Journal name Lipids in health and disease
Volume number 9
Start page 1
End page 8
Publisher BioMed Central
Place of publication London, England
Publication date 2010-06-04
ISSN 1476-511X
Summary Background : Lipid droplet (LD) formation and size regulation reflects both lipid influx and efflux, and is central in the regulation of adipocyte metabolism, including adipokine secretion. The length and degree of dietary fatty acid (FA) unsaturation is implicated in LD formation and regulation in adipocytes. The aims of this study were to establish the impact of eicosapentaenoic acid (EPA; C20:5n-3) in comparison to SFA (STA; stearic acid, C18:0) and MUFA (OLA; oleic acid, C18:1n-9) on 3T3-L1 adipocyte LD formation, regulation of genes central to LD function and adipokine responsiveness. Cells were supplemented with 100 μM FA during 7-day differentiation.

Results : EPA markedly reduced LD size and total lipid accumulation, suppressing PPARγ, Cidea and D9D/SCD1 genes, distinct from other treatments. These changes were independent of alterations of lipolytic genes, as both EPA and STA similarly elevated LPL and HSL gene expressions. In response to acute lipopolysaccharide exposure, EPA-differentiated adipocytes had distinct improvement in inflammatory response shown by reduction in monocyte chemoattractant protein-1 and interleukin-6 and elevation in adiponectin and leptin gene expressions.

Conclusions : This study demonstrates that EPA differentially modulates adipogenesis and lipid accumulation to suppress LD formation and size. This may be due to suppressed gene expression of key proteins closely associated with LD function. Further analysis is required to determine if EPA exerts a similar influence on LD formation and regulation in-vivo.
Notes This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Language eng
Field of Research 110101 Medical Biochemistry: Amino Acids and Metabolites
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2010, Manickam et al.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30029847

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.