Solubility and Stability of cytochrome c in hydrated ionic liquids : effect of oxo acid residues and kosmotropicity

Fujita, Kyoko, MacFarlane, Douglas R., Forsyth, Maria, Yoshizawa-Fujita, Masahiro, Murata, Kenichi, Nakamura, Nobuhumi and Ohno, Hiroyuki 2007, Solubility and Stability of cytochrome c in hydrated ionic liquids : effect of oxo acid residues and kosmotropicity, Biomacromolecules, vol. 8, no. 7, pp. 2080-2086.

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Title Solubility and Stability of cytochrome c in hydrated ionic liquids : effect of oxo acid residues and kosmotropicity
Formatted title Solubility and Stability of cytochrome c in hydrated ionic liquids : effect of oxo acid residues and kosmotropicity
Author(s) Fujita, Kyoko
MacFarlane, Douglas R.
Forsyth, Maria
Yoshizawa-Fujita, Masahiro
Murata, Kenichi
Nakamura, Nobuhumi
Ohno, Hiroyuki
Journal name Biomacromolecules
Volume number 8
Issue number 7
Start page 2080
End page 2086
Publisher American Chemical Society
Place of publication Washington, D.C.
Publication date 2007-07
ISSN 1525-7797
1526-4602
Summary Hydrated ionic liquids (ILs) were prepared by adding appropriate amounts of water to hydrophilic ILs. Some hydrated ILs show excellent solubilizing ability for proteins, keeping the basic properties of ILs. The solubility of cytochrome c (cyt c) depended on the structure of the component ions. When component anions have oxo acid residues, the resulting hydrated ILs solubilize cyt c quite well. In such hydrated ILs, the structure and activity of cyt c is influenced by the kosmotropicity of the component ions. We synthesized ILs from various ions having different kosmotropicity, including dihydrogen phosphate (dhp), dibutylphosphate, acetate, lactate, and methanesulfonate as anions. The activity of the dissolved cyt c depends on the permutations of kosmotropicity of the component ions. cyt c shows no structural change and retains its activity when dissolved in the hydrated choline dhp, which is an excellent combination of chaotropic cation and kosmotropic anion. Furthermore, cyt c dissolved in the hydrated choline dhp remained in a native state and was active after 18 months of storage at room temperature.
Language eng
Field of Research 039999 Chemical Sciences not elsewhere classified
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2007, American Chemical Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30030157

Document type: Journal Article
Collection: Institute for Technology Research and Innovation
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