N-Acetylcysteine infusion does not affect glucose disposal during prolonged moderate-intensity exercise in humans

Merry, Troy L., Wadley, Glen D., Stathis, Christos G., Garnham, Andrew P., Rattigan, Stephen, Hargreaves, Mark and McConell, Glenn K. 2010, N-Acetylcysteine infusion does not affect glucose disposal during prolonged moderate-intensity exercise in humans, The Journal of Physiology, vol. 588, no. 9, pp. 1623-1634.

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Title N-Acetylcysteine infusion does not affect glucose disposal during prolonged moderate-intensity exercise in humans
Author(s) Merry, Troy L.
Wadley, Glen D.
Stathis, Christos G.
Garnham, Andrew P.
Rattigan, Stephen
Hargreaves, Mark
McConell, Glenn K.
Journal name The Journal of Physiology
Volume number 588
Issue number 9
Start page 1623
End page 1634
Total pages 12
Publisher Wiley-Blackwell Publishing
Place of publication Oxford, England
Publication date 2010-05-01
ISSN 0022-3751
1469-7793
Summary There is evidence that reactive oxygen species (ROS) signalling is required for normal increases in glucose uptake during contraction of isolated mouse skeletal muscle, and that AMP-activated protein kinase (AMPK) is involved. The aim of this study was to determine whether ROS signalling is involved in the regulation of glucose disposal and AMPK activation during moderate-intensity exercise in humans. Nine healthy males completed 80 min of cycle ergometry at 62 ± 1 of peak oxygen consumption ( . A 6,6-2H-glucose tracer was infused at rest and during exercise, and in a double-blind randomised cross-over design, N-acetylcysteine (NAC) or saline (CON) was co-infused. NAC was infused at 125 mg kg?1h?1for 15 min and then at 25 mg kg?1h?1for 20 min before and throughout exercise. NAC infusion elevated plasma NAC and cysteine, and muscle NAC and cysteine concentrations during exercise. Although neither NAC infusion nor exercise significantly affected muscle reduced or oxidised glutathione (GSH or GSSG) concentration (P> 0.05), S-glutathionylation (an indicator of oxidative stress) of a protein band of ?270 kDa was increased ?3-fold with contraction and this increase was prevented by NAC infusion. Despite this, exercised-induced increases in tracer determined glucose disposal, plasma lactate, plasma non-esterified fatty acids (NEFAs), and decreases in plasma insulin were not affected by NAC infusion. In addition, skeletal muscle AMPK? and acetyl-CoA carboxylase-? (ACC?) phosphorylation increased during exercise by ?3- and ?6-fold (P< 0.05), respectively, and this was not affected by NAC infusion. Unlike findings in mouse muscle ex vivo, NAC does not attenuate skeletal muscle glucose disposal or AMPK activation during moderate-intensity exercise in humans.
Language eng
Field of Research 110602 Exercise Physiology
Socio Economic Objective 920104 Diabetes
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2010
Copyright notice ©2010, Wiley-Blackwell Publishing
Persistent URL http://hdl.handle.net/10536/DRO/DU:30030962

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