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Epithelial cell folate is an accurate marker when compared with whole tissue biopsy folate for examining the role of folate status in colorectal cancer

O'Reilly, S. L. 2006, Epithelial cell folate is an accurate marker when compared with whole tissue biopsy folate for examining the role of folate status in colorectal cancer, in NSA 2006 : Nutrition through the Life Cycle : Proceedings of the Nutrition Society of Australia, HEC Press, Melbourne, Vic., pp. S127-S127.

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Title Epithelial cell folate is an accurate marker when compared with whole tissue biopsy folate for examining the role of folate status in colorectal cancer
Author(s) O'Reilly, S. L.ORCID iD for O'Reilly, S. L. orcid.org/0000-0003-3547-6634
Conference name Nutrition Society of Australia. Scientific Meeting (30th :2006 : Sydney, N.S.W.)
Conference location Sydney, N.S.W.
Conference dates 29 Nov.-2 Dec. 2006
Title of proceedings NSA 2006 : Nutrition through the Life Cycle : Proceedings of the Nutrition Society of Australia
Publication date 2006
Start page S127
End page S127
Total pages 1
Publisher HEC Press
Place of publication Melbourne, Vic.
Keyword(s) epithelial cell
colorectal cancer
folate
methodology
Summary Background – Epidemiological studies have shown low folate status is associated with colorectal cancer. Colonic tissue folate levels at different stages of cancer development should give important information, but different methodologies to extract the colonic tissue folates have been used. This has hampered progress in defining the relationship between systemic and tissue folate levels.
Objective – To evaluate two methods of colonic tissue preparation for estimation of total folate content.
Design – Whole tissue punch biopsy samples were obtained from the descending colon of 31individuals following a normal colonoscopy. Blood samples were obtained for the determination of plasma homocysteine (Hcy), red cell folate (RCF), methylenetetrahydrofolate reductase 677C>T genotype, and serum vitamin B12 and folate. Colonic tissue folate was measured both in washed whole tissue biopsies and in epithelial cells isolated from tissue biopsies.
Outcomes - Whole biopsy and epithelial cell folate concentrations were significantly correlated (R=.375; P=.038). Hcy was inversely correlated with both measures (R=-.365; P=.043 and R=-.364; P=.044 respectively). RCF was significantly correlated with isolated epithelial cell folate (R=.477; P=.007) but not with whole tissue biopsy folate (R=.264; P=.151). There were no significant associations between serum and colonic folate in this study.
Conclusion - Both methods are useful for comparing systemic and localised tissue folate status but epithelial cells may provide more reliable data.


ISSN 0964-7058
1440-6047
Language eng
Field of Research 111103 Nutritional Physiology
Socio Economic Objective 920102 Cancer and Related Disorders
HERDC Research category E3.1 Extract of paper
HERDC collection year 2006
Copyright notice ©2001-2007, HEC PRESS
Persistent URL http://hdl.handle.net/10536/DRO/DU:30031263

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Created: Fri, 19 Nov 2010, 13:14:41 EST by Sharleen O'reilly

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